In Vitro Evaluation of Synergistic Essential Oils Combination for Enhanced Antifungal Activity against Candida spp.

被引:3
|
作者
Hleba, Lukas [1 ]
Hlebova, Miroslava [2 ]
Charousova, Ivana [3 ]
机构
[1] Slovak Univ Agr, Inst Biotechnol, Fac Biotechnol & Food Sci, Tr A Hlinku 2, Nitra 94976, Slovakia
[2] Univ SS Cyril & Methodius, Inst Biol & Biotechnol, Fac Nat Sci, Nam J Herdu 2, SK-91701 Trnava, Slovakia
[3] Unilabs Slovensko Sro, Clin Microbiol Lab, J Bellu 66, SK-03495 Likavka, Slovakia
来源
LIFE-BASEL | 2024年 / 14卷 / 06期
关键词
Candida spp; essential oils; synergy effect; antifungal activity; in vitro; TEA TREE OIL; ANTIMICROBIAL ACTIVITY; ALBICANS; AGENTS; MODE; L; IDENTIFICATION; ASPERGILLUS; RESISTANCE; BIOFILM;
D O I
10.3390/life14060693
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
In recent years, a significant number of infections have been attributed to non-albicidal Candida species (NAC), mainly due to the increasing resistance of NAC to antifungal agents. As only a few antifungal agents are available (azoles, echinocandins, polyenes, allylamines and nucleoside analogues), it is very important to look for possible alternatives to inhibit resistant fungi. One possibility could be essential oils (EOs), which have been shown to have significant antifungal and antibacterial activity. Therefore, in this study, the efficacy of 12 EOs and their combinations was evaluated against four yeasts of the genus Candida (C. albicas, C. glabrata, C. tropicalis and C. parapsilosis). GC-MS and GC-MS FID techniques were used for the chemical analysis of all EOs. VITEK-2XL was used to determine the antifungal susceptibility of the tested Candida spp. strains. The agar disc diffusion method was used for primary screening of the efficacy of the tested EOs. The broth dilution method was used to determine the minimum inhibitory concentrations (MICs) of the most potent EOs. After MIC cultivation, the minimum fungicidal concentration (MFC) was determined on Petri dishes (60 mm). The synergistic effect of combined EOs was evaluated using the checkerboard method and expressed as a fractional inhibitory concentration index (FICI). The results showed that ginger > ho-sho > absinth > dill > fennel > star anise > and cardamom were the most effective EOs. For all Candida species tested, the synergy was mainly observed in these combinations: ginger/fennel for C. albicans FICI 0.25 and C. glabrata, C. tropicalis and C. parapsilosis FICI 0.5 and absinth/fennel for C. albicans FICI 0.3125, C. tropicalis FICI 0.3125 and C. parapsilosis FICI 0.375. Our results suggest that the resistance of fungal pathogens to available antifungals could be reduced by combining appropriate EOs.
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页数:17
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