Sensors;
Antibodies;
Nanobioscience;
Electrodes;
Proteins;
Monitoring;
Hydrogels;
Chemical and biological sensors;
antibiofouling;
electrochemical biosensor (EB);
kidney transplant rejection;
nanocomposite;
D O I:
10.1109/LSENS.2024.3399604
中图分类号:
TM [电工技术];
TN [电子技术、通信技术];
学科分类号:
0808 ;
0809 ;
摘要:
Diagnosing acute rejection of kidney transplant remains challenging as standard practice relies on monitoring serum creatinine levels and performing biopsies. The former lacks sensitivity, while the latter is invasive. Monitoring Chemokine IP-10 (or CXCL10) in patient samples has shown promising results in addressing these bottlenecks but is measured with expensive and time-consuming protocols, such as enzyme-linked immunosorbent assay. This does not allow for an individualized approach. The present work describes the development of a novel screen-printed electrode-based electrochemical biosensor (EB) for IP-10 detection using an electroactive antibiofouling hydrogel. The electrode coating is based on glutaraldehyde (GA)-crosslinked 3-D nanostructured bovine serum albumin (BSA) hydrogel, whose pores are filled with a highly conductive Ti3C2Tx MXene. The formulation is water-based and avoids the use of toxic solvents. The crosslinking mechanism, antibiofouling characteristics, and electroactivity were characterized by UV-VIS spectroscopy, contact angle measurement, and cyclic voltammetry tests, respectively. The antibody-functionalized MXene/BSA/GA nanocomposite-based sensor allows the detection of IP-10 spiked in human serum by achieving an LOD of 3.3 pg/ml with a linear range across 1-200 pg/ml and a response time of 30 min. The letter paves the way toward developing a highly specific multiplexed chemokine profiling platform for point-of-care diagnostics applications to monitor kidney transplant rejection.
机构:
Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao, Peoples R China
Shihezi Univ, Coll Anim Sci & Technol, Shihezi, Xinjiang, Peoples R China
Kashi Jinhuitong Ind Dev Co Ltd, Kashi, Peoples R ChinaQingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao, Peoples R China
Chen, Lihua
Zhao, Shiyi
论文数: 0引用数: 0
h-index: 0
机构:
Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao, Peoples R ChinaQingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao, Peoples R China
Zhao, Shiyi
Liu, Mingchao
论文数: 0引用数: 0
h-index: 0
机构:
Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao, Peoples R ChinaQingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao, Peoples R China
Liu, Mingchao
Wu, Peng
论文数: 0引用数: 0
h-index: 0
机构:
Shihezi Univ, Coll Anim Sci & Technol, Shihezi, Xinjiang, Peoples R ChinaQingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao, Peoples R China
Wu, Peng
Chen, Chuangfu
论文数: 0引用数: 0
h-index: 0
机构:
Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao, Peoples R China
Shihezi Univ, Coll Anim Sci & Technol, Shihezi, Xinjiang, Peoples R ChinaQingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao, Peoples R China