Label-Free Electrochemical Immunosensor for Monitoring Kidney Transplant Rejection With Electroactive Antibiofouling Hydrogel

被引:1
|
作者
Gupta, Rohit [1 ,2 ]
Salaris, Nikolaos [1 ,2 ]
Kalkal, Ashish [1 ,2 ]
Mandal, Priya [1 ,2 ]
Balabani, Stavroula [2 ,3 ]
Motallebzadeh, Reza [4 ]
Tiwari, Manish K. [1 ,2 ]
机构
[1] UCL, Dept Mech Engn, Nanoengn Syst Lab, London, England
[2] UCL, Wellcome EPSRC Ctr Intervent & Surg Sci, London W1W 7TS, England
[3] UCL, Dept Mech Engn, FluME, London WC1E 7JE, England
[4] UCL, Dept Surg, London NW3 2PF, England
基金
英国工程与自然科学研究理事会;
关键词
Sensors; Antibodies; Nanobioscience; Electrodes; Proteins; Monitoring; Hydrogels; Chemical and biological sensors; antibiofouling; electrochemical biosensor (EB); kidney transplant rejection; nanocomposite;
D O I
10.1109/LSENS.2024.3399604
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
Diagnosing acute rejection of kidney transplant remains challenging as standard practice relies on monitoring serum creatinine levels and performing biopsies. The former lacks sensitivity, while the latter is invasive. Monitoring Chemokine IP-10 (or CXCL10) in patient samples has shown promising results in addressing these bottlenecks but is measured with expensive and time-consuming protocols, such as enzyme-linked immunosorbent assay. This does not allow for an individualized approach. The present work describes the development of a novel screen-printed electrode-based electrochemical biosensor (EB) for IP-10 detection using an electroactive antibiofouling hydrogel. The electrode coating is based on glutaraldehyde (GA)-crosslinked 3-D nanostructured bovine serum albumin (BSA) hydrogel, whose pores are filled with a highly conductive Ti3C2Tx MXene. The formulation is water-based and avoids the use of toxic solvents. The crosslinking mechanism, antibiofouling characteristics, and electroactivity were characterized by UV-VIS spectroscopy, contact angle measurement, and cyclic voltammetry tests, respectively. The antibody-functionalized MXene/BSA/GA nanocomposite-based sensor allows the detection of IP-10 spiked in human serum by achieving an LOD of 3.3 pg/ml with a linear range across 1-200 pg/ml and a response time of 30 min. The letter paves the way toward developing a highly specific multiplexed chemokine profiling platform for point-of-care diagnostics applications to monitor kidney transplant rejection.
引用
收藏
页码:1 / 4
页数:4
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