Technical considerations for placental tissue processing and the subsequent impact on genome-wide DNA methylation analysis

被引：0

作者：

Kocher, Kristen M. ^{[1
,4
]}

Ngwa, Julius ^{[1
]}

Kapse, Kushal J. ^{[1
]}

Bhattacharya, Surajit ^{[3
]}

Rossi, Christopher ^{[2
]}

Delot, Emmanuele ^{[3
,4
]}

duPlessis, Adre ^{[4
,5
]}

Limperopoulos, Catherine ^{[1
,4
]}

Andescavage, Nickie ^{[1
,4
,6
]}

机构：

[1] Childrens Natl Hosp, Developing Brain Inst, Childrens Res Inst, Ctr Prenatal Neonatal & Maternal Hlth Res, Washington, DC 20010 USA

[2] Childrens Natl Hosp, Dept Pathol & Lab Med, Washington, DC USA

[3] Childrens Natl Hosp, Childrens Res Inst, Ctr Genet Med Res, Washington, DC USA

[4] George Washington Univ, Dept Pediat, Washington, DC 20052 USA

[5] Childrens Natl Hosp, Zickler Family Prenatal Pediat Inst, Washington, DC USA

[6] Childrens Natl Hosp, Div Neonatol, Washington, DC 20010 USA

来源：

CLINICAL EPIGENETICS | 2024年 / 16卷 / 01期

关键词：

Placenta; DNA methylation; Epigenetic aging; FFPE;

D O I：

10.1186/s13148-024-01741-8

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

To assess the impact of postnatal processing on placental DNA methylation, array data from flash-frozen placental tissue was compared to perfluorocarbon-immersed and formalin-fixed paraffin-embedded placental tissue. We observed that tissue exposed to perfluorocarbon showed no significant DNA methylation differences when compared to unprocessed tissue, while formalin processing altered the quality and reliability of the data produced on the DNA methylation array platform. Placental DNA methylation allows for the study of gene-environment interactions that influence the fetal environment and development. Our study highlights that placental post-processing techniques must be considered in the evaluation and interpretation of epigenetic studies.

引用

页数：8

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