High pre-Delta and early-Omicron SARS-CoV-2 seroprevalence detected in dried blood samples from Kinshasa (DR Congo)

被引:1
作者
de Aguirre, Paula Martinez [1 ]
Carlos, Silvia [2 ,3 ]
Pina-Sanchez, Manuel [1 ]
Mbikayi, Samclide [4 ]
Burgueno, Eduardo [4 ]
Tendobi, Celine [4 ]
Chiva, Luis [1 ]
Holguin, Africa [5 ]
Reina, Gabriel [1 ]
机构
[1] Clin Univ Navarra, Pamplona, Spain
[2] Univ Navarra, Dept Prevent Med & Publ Hlth, C Irunlarrea 1, Pamplona 31008, Spain
[3] Inst Invest Sanitaria Navarra IdiSNA, Irunlarrea 3, Pamplona, Spain
[4] Ctr Hosp Monkole, Kinshasa, DEM REP CONGO
[5] Hosp Ramon & Cajal, IRYCIS, RITIP, CIBERESP,Lab Epidemiol Mol VIH 1, Madrid, Spain
关键词
chemiluminescence; COVID-19; DBS; SARS-CoV-2; seroprevalence; sub-Saharan Africa; COVID-19; INFECTION; PREVALENCE; ANTIBODIES; DIAGNOSIS; UGANDA; IMPACT;
D O I
10.1002/jmv.29529
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Studies on the impact of the COVID-19 pandemic in sub-Saharan Africa have yielded varying results, although authors universally agree the real burden surpasses reported cases. The primary objective of this study was to determine SARS-CoV-2 seroprevalence among patients attending Monkole Hospital in Kinshasa (D.R. Congo). The secondary objective was to evaluate the analytic performance of two chemiluminescence platforms: Elecsys (R) (Roche) and VirClia (R) (Vircell) on dried blood spot samples (DBS). The study population (N=373) was recruited in two stages: a mid-2021 blood donor cohort (15.5% women) and a mid-2022 women cohort. Crude global seroprevalence was 61% (53.9%-67.8%) pre-Delta in 2021 and 90.2% (84.7%-94.2%) post-Omicron in 2022. Anti-spike (S) antibody levels significantly increased from 53.1 (31.8-131.3) U/mL in 2021 to 436.5 (219.3-950.5) U/mL in 2022 and were significantly higher above 45 years old in the 2022 population. Both platforms showed good analytic performance on DBS samples: sensitivity was 96.8% for IgG (antiN/S) (93.9%-98.5%) and 96.0% (93.0%-98.0%) for anti-S quantification. These results provide additional support for the notion that exposure to SARS-CoV-2 is more widespread than indicated by case-based surveillance and will be able to guide the pandemic response and strategy moving forward. Likewise, this study contributes evidence to the reliability of DBS as a tool for serological testing and diagnosis in resource-limited settings.
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共 68 条
[1]   High SARS-CoV-2 seroprevalence in Lagos, Nigeria with robust antibody and cellular immune responses [J].
Akanmu, Sulaimon ;
Herrera, Bobby Brooke ;
Chaplin, Beth ;
Ogunsola, Sade ;
Osibogun, Akin ;
Onawoga, Fatima ;
John-Olabode, Sarah ;
Akase, Iorhen E. ;
Nwosu, Augustina ;
Hamel, Donald J. ;
Chang, Charlotte A. ;
Kanki, Phyllis J. .
JOURNAL OF CLINICAL VIROLOGY PLUS, 2023, 3 (03)
[2]   Seasonal human coronavirus antibodies are boosted upon SARS-CoV-2 infection but not associated with protection [J].
Anderson, Elizabeth M. ;
Goodwin, Eileen C. ;
Verma, Anurag ;
Arevalo, Claudia P. ;
Bolton, Marcus J. ;
Weirick, Madison E. ;
Gouma, Sigrid ;
McAllister, Christopher M. ;
Christensen, Shannon R. ;
Weaver, JoEllen ;
Hicks, Philip ;
Manzoni, Tomaz B. ;
Oniyide, Oluwatosin ;
Ramage, Holly ;
Mathew, Divij ;
Baxter, Amy E. ;
Oldridge, Derek A. ;
Greenplate, Allison R. ;
Wu, Jennifer E. ;
Alanio, Cecile ;
D'Andrea, Kurt ;
Kuthuru, Oliva ;
Dougherty, Jeanette ;
Pattekar, Ajinkya ;
Kim, Justin ;
Han, Nicholas ;
Apostolidis, Sokratis A. ;
Huang, Alex C. ;
Vella, Laura A. ;
Kuri-Cervantes, Leticia ;
Pampena, M. Betina ;
Betts, Michael R. ;
Wherry, E. John ;
Meyer, Nuala J. ;
Cherry, Sara ;
Bates, Paul ;
Rader, Daniel J. ;
Hensley, Scott E. .
CELL, 2021, 184 (07) :1858-+
[3]  
Audu RA, 2022, PLOS GLOB PUBL HLTH, V2, DOI 10.1371/journal.pgph.0000363
[4]   High seroprevalence of Immunoglobulin G (IgG) and IgM antibodies to SARS-CoV-2 in asymptomatic and symptomatic individuals amidst vaccination roll-out in western Kenya [J].
Awandu, Shehu Shagari ;
Ochieng, Alfred Ochieng ;
Onyango, Benson ;
Magwanga, Richard Odongo ;
Were, Pamela ;
Ochung', Angeline Atieno ;
Okumu, Fredrick ;
Oloo, Marceline Adhiambo ;
Katieno, Jim Seth ;
Lidechi, Shirley ;
Ogutu, Fredrick ;
Awuor, Dorothy ;
Kirungu, Joy Nyangasi ;
Orata, Francis ;
Achieng, Justine ;
Oure, Bonface ;
Nyunja, Regina ;
Muok, Eric M. O. ;
Munga, Stephen ;
Estambale, Benson .
PLOS ONE, 2022, 17 (12)
[5]   Estimating global, regional, and national daily and cumulative infections with SARS-CoV-2 through Nov 14, 2021: a statistical analysis [J].
Barber, Ryan M. ;
Sorensen, Reed J. D. ;
Pigott, David M. ;
Bisignano, Catherine ;
Carter, Austin ;
Amlag, Joanne O. ;
Collins, James K. ;
Abbafati, Cristiana ;
Adolph, Christopher ;
Allorant, Adrien ;
Aravkin, Aleksandr Y. ;
Bang-Jensen, Bree L. ;
Castro, Emma ;
Chakrabarti, Suman ;
Cogen, Rebecca M. ;
Combs, Emily ;
Comfort, Haley ;
Cooperrider, Kimberly ;
Dai, Xiaochen ;
Daoud, Farah ;
Deen, Amanda ;
Earl, Lucas ;
Erickson, Megan ;
Ewald, Samuel B. ;
Ferrari, Alize J. ;
Flaxman, Abraham D. ;
Frostad, Joseph Jon ;
Fullman, Nancy ;
Giles, John R. ;
Guo, Gaorui ;
He, Jiawei ;
Helak, Monika ;
Hulland, Erin N. ;
Huntley, Bethany M. ;
Lazzar-Atwood, Alice ;
LeGrand, Kate E. ;
Lim, Stephen S. ;
Lindstrom, Akiaja ;
Linebarger, Emily ;
Lozano, Rafael ;
Magistro, Beatrice ;
Malta, Deborah Carvalho ;
Mansson, Johan ;
Herrera, Ana M. Mantilla ;
Mokdad, Ali H. ;
Monasta, Lorenzo ;
Naghavi, Mohsen ;
Nomura, Shuhei ;
Odell, Christopher M. ;
Olana, Latera Tesfaye .
LANCET, 2022, 399 (10344) :2351-2380
[6]   HIV-1 diagnosis using dried blood spots from patients in Kinshasa, DRC: a tool to detect misdiagnosis and achieve World Health Organization 2030 targets [J].
Barquin, David ;
Ndarabu, Adolphe ;
Carlos, Silvia ;
Fernandez-Alonso, Mirian ;
Rubio-Garrido, Marina ;
Makonda, Benit ;
Holguin, Africa ;
Reina, Gabriel .
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2021, 111 :253-260
[7]  
Bingham J., 2022, RES SQ, DOI [10.21203/rs.3.rs-1687679/v1, DOI 10.21203/RS.3.RS-1687679/V1]
[8]   SARS-CoV-2 seroprevalence among blood donors in Uganda: 2019-2022 [J].
Bloch, Evan M. M. ;
Kyeyune, Dorothy ;
White, Jodie L. L. ;
Ddungu, Henry ;
Ashokkumar, Swetha ;
Habtehyimer, Feben ;
Baker, Owen ;
Kasirye, Ronnie ;
Patel, Eshan U. U. ;
Grabowski, M. Kate ;
Musisi, Ezra ;
Moses, Khan ;
Hume, Heather A. A. ;
Lubega, Irene ;
Shrestha, Ruchee ;
Motevalli, Mahnaz ;
Fernandez, Reinaldo E. E. ;
Reynolds, Steven J. J. ;
Redd, Andrew D. D. ;
Musana, Hellen Wambongo ;
Dhabangi, Aggrey ;
Ouma, Joseph ;
Eroju, Priscilla ;
de Lange, Telsa ;
Fowler, Mary Glenn ;
Musoke, Philippa ;
Stramer, Susan L. L. ;
Whitby, Denise ;
Zimmerman, Peter A. A. ;
McCullough, Jeffrey ;
Sachithanandham, Jaiprasath ;
Pekosz, Andrew ;
Goodrich, Raymond ;
Quinn, Thomas C. C. ;
Ness, Paul M. M. ;
Laeyendecker, Oliver ;
Tobian, Aaron A. R. .
TRANSFUSION, 2023, 63 (07) :1354-1365
[9]   Seroprevalence of Antibodies to SARS-CoV-2 in Rural Households in Eastern Uganda, 2020-2022 [J].
Briggs, Jessica ;
Takahashi, Saki ;
Nayebare, Patience ;
Cuu, Gloria ;
Rek, John ;
Zedi, Maato ;
Kizza, Timothy ;
Arinaitwe, Emmanuel ;
Nankabirwa, Joaniter I. ;
Kamya, Moses ;
Jagannathan, Prasanna ;
Jacobson, Karen ;
Rosenthal, Philip J. ;
Dorsey, Grant ;
Greenhouse, Bryan ;
Ssewanyana, Isaac ;
Rodriguez-Barraquer, Isabel .
JAMA NETWORK OPEN, 2023, 6 (02) :E2255978
[10]   COVID-19 in the 47 countries of the WHO African region: a modelling analysis of past trends and future patterns [J].
Cabore, Joseph Waogodo ;
Karamagi, Humphrey Cyprian ;
Kipruto, Hillary Kipchumba ;
Mungatu, Joseph Kyalo ;
Asamani, James Avoka ;
Droti, Benson ;
Titi-Ofei, Regina ;
Seydi, Aminata Binetou Wahebine ;
Kidane, Solyana Ngusbrhan ;
Balde, Thierno ;
Gueye, Abdou Salam ;
Makubalo, Lindiwe ;
Moeti, Matshidiso R. .
LANCET GLOBAL HEALTH, 2022, 10 (08) :E1099-E1114