Antibacterial Activity of the Allium sativum Crude Extract against Methicillin-resistant Staphylococcus aureus

Garlic contains various components, such as allicin, ajoenes, and allyl sulfides, which are organosulfur compounds. It exhibits antimicrobial activities in all forms, including garlic powder, garlic extract, and garlic oil. Additionally, it exerts anti-inflammatory, antibacterial, antibiofilm, and antifungal effects. The drastic increase in drug-resistant organisms worldwide, especially methicillin-resistant Staphylococcus aureus (MRSA) strains, has led to treatment failure in various infectious diseases. To overcome this issue, we aimed to assess the antibacterial activity of garlic extract against MRSA and explore its action mechanism against penicillin-binding proteins (PBPs) in this study. Mueller-Hinton agar was used for the agar well diffusion test. Moreover, bactericidal activity was determined using the Mueller-Hinton agar and spot tests. Molecular docking was performed using AUTODOCK to evaluate the PBP-binding ability of allicin. A wide zone of inhibition was observed for MRSA, and molecular docking revealed a strong interaction between PBP3 of S. aureus and allicin. Overall, our findings revealed the antibacterial activity of garlic extract against MRSA, a prevalent gram-positive coccus causing healthcare-associated infections. In addition to its affordability, the beneficial effects of garlic suggest its therapeutic potential for MRSA treatment in combination with cefoxitin.