A grafting approach for nisin-chitosan bio-based antibacterial films: preparation and characterization

Nisin is a bacteriocin produced by Gram-positive lactic acid bacterium, Lactococcus lactis and currently recognized in the Generally Recogn & imath;zed as Safe (GRAS) category due to its non-toxicity. Herein, nisin has been grafted to chitosan structure to obtain natural bio-active films with enhanced antibacterial activity. Grafting was performed using ethyl ester lysine diisocyanate and dimer fatty acid-based diisocyanate (DDI); two different close to fully bio-based diisocyanates and Disuccinimidyl suberate; a homo-bifunctional molecule acting as a crosslinker between amino groups. The grafting process allowed the chemical immobilization of nisin to chitosan structure. Physicochemical characterization studies showed the successful grafting of nisin. The antibacterial activity against Staphylococcus aureus was evident for all nisin modified chitosan films and best pronounced when DDI was used as a crosslinker with a maximum zone of inhibition of similar to 13 mm. All nisin grafted chitosan films were cytocompatible and the cell viability of L929 fibroblasts were >80% pointing out the non-toxic structure. Considering the results of the presented study, bio-based diisocyanates and homo-bifunctional crosslinkers are effective molecules in synthesis of nisin grafted chitosan structures and the new chitosan based antibacterial biopolymers obtained after nisin modification come forward as promising non-toxic and bioactive candidates to be applied in medical devices, implants, and various food coating products.