Prevalence and Molecular Characteristics of Enterococci Isolated from Clinical Bovine Mastitis Cases in Ningxia

被引：4

作者：

Liu, Jing ^{[1
]}

Liang, Zeyi ^{[1
]}

Zhongla, Maocao ^{[2
]}

Wang, Hongsheng ^{[3
]}

Sun, Xu ^{[4
]}

Zheng, Juanshan ^{[1
]}

Ding, Xuezhi ^{[1
,3
,5
]}

Yang, Feng ^{[1
,5
]}

机构：

[1] Chinese Acad Agr Sci, Lanzhou Inst Husb & Pharmaceut Sci, Key Lab New Anim Drug Project Gansu Prov, Key Lab Vet Pharmaceut Discovery, Lanzhou, Peoples R China

[2] Gannan Anim Dis Prevent & Control Ctr, Hezuo, Peoples R China

[3] Xiangyang Vocat & Tech Coll, Xiangyang, Peoples R China

[4] Lanzhou Univ Technol, Coll Life Sci & Engn, Lanzhou, Peoples R China

[5] Lanzhou Inst Husb & Pharmaceut Sci, Chinese Acad Agr Sci, 335 Jiangouyan, Lanzhou 730050, Gansu, Peoples R China

来源：

INFECTION AND DRUG RESISTANCE | 2024年 / 17卷

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

bovine mastitis; enterococci; antimicrobial resistance; virulence; ANTIMICROBIAL RESISTANCE; ANTIBIOTIC-RESISTANCE; VIRULENCE FACTORS; GENETIC-CHARACTERIZATION; TETRACYCLINE RESISTANCE; BIOFILM FORMATION; DAIRY-COWS; SPP; FAECALIS; FOOD;

D O I：

10.2147/IDR.S461587

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Purpose: This study aimed to investigate the prevalence and genetic characterization of enterococcal isolates (Enterococcus faecalis, Enterococcus faecium and Enterococcus hirae) isolated from clinical bovine mastitis cases in Ningxia, China. Patients and Methods: The enterococci were identified by 16S rRNA amplification and sequencing. Antimicrobial resistance was determined by disc diffusion method. Virulence and antimicrobial resistance genes were detected by PCR assays. Results: Overall, 198 enterococcal isolates were identified from 2897 mastitis samples, including 137 (4.7%) E. faecalis, 50 (1.7%) E. faecium and 11 (0.4%) E. hirae. E. faecalis, E. faecium and E. hirae isolates showed high resistance to tetracycline (92.7%, 68.0%, 90.9%), followed by erythromycin (86.9%, 76.0%, 72.7%). The multidrug-resistant strains of E. faecalis and E. faecium were 29 (21.2%) and 13 (26.0%), respectively. The resistance of E. faecalis, E. faecium and E. hirae isolates to tetracycline is mainly attributed to the presence of tetL (alone or combined with tetM and/or tetK), the erythromycin resistance to ermB (alone or combined with ermC and/or ermA). Moreover, cpd (94.2%), gelE (77.4%), efaAfs (93.4%), and esp (79.6%) were the most common virulence genes in E. faecalis. In E. faecium, except for the gene efaAfs (82.0%), other virulence genes are rarely found. Only two strains of E. hirae carrying asa1 gene were detected. Conclusion: The results of this study can provide a reference for the prevention and treatment of bovine mastitis caused by enterococci.

引用

页码：2121 / 2129

页数：9

共 77 条

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