SNP-based h igh-resolution typing of Chlamydia psittaci from humans and wild birds in Sweden: circulation of the Mat116 genotype reveals the transmission mode to humans

被引：2

作者：

Herrmann, Bjorn ^{[1
,2
]}

Aaziz, Rachid

Kaden, Rene ^{[1
,4
]}

Riedel, Hilde M. ^{[1
,2
]}

Sporndly-Nees, Ellinor ^{[5
]}

Sandelin, Lisa Labbe ^{[6
,7
]}

Laroucau, Karine ^{[3
]}

机构：

[1] Uppsala Univ Hosp, Dept Clin Microbiol, SE-75185 Uppsala, Sweden

[2] Uppsala Univ, Dept Med Sci, Clin Microbiol, SE-75185 Uppsala, Sweden

[3] Paris Est Univ, Bacterial Zoonosis Unit, Lab Anim Hlth, Anses, F-94706 Maisons Alfort, France

[4] Sci Life Lab, Clin Genom Uppsala, SE-75185 Uppsala, Sweden

[5] Natl Vet Inst SVA, Dept Pathol & Wildlife Dis, SE-75189 Uppsala, Sweden

[6] Dept Communicable Dis & Dis Control, SE-39126 Kalmar, Kalmar, Sweden

[7] Linnaeus Univ, Dept Biol & Environm Sci, SE-39182 Kalmar, Sweden

来源：

MICROBES AND INFECTION | 2024年 / 26卷 / 03期

关键词：

Chlamydia psittaci; Sweden; Psittacosis; Genotyping; MLST; High resolution-PCR; CHLAMYDOPHILA-PSITTACI; FERAL PIGEONS; FULMARS;

D O I：

10.1016/j.micinf.2023.105251

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The incidence of Chlamydia psittaci respiratory tract infections in humans has increased in Sweden in recent years. This study aimed to identify the transmission route by genotyping C. psittaci from infected humans and birds. 42 human C. psittaci samples and 5 samples from C. psittaci-infected birds were collected. Genotyping was performed using ompA sequencing, Multi-locus sequence typing, and/or SNP-based high-resolution melting-PCR. Epidemiological data was also collected, and a phylogenetic analysis was conducted. Analysis of ompA provided limited resolution, while the SNP-based PCR analysis successfully detected the Mat116 genotype in 3/5 passerine birds and in 26/29 human cases, indicating a high prevalence of this genotype in the human population. These cases were associated with contact with wild birds, mainly through bird feeding during winter or other outdoor exposure. Human cases caused by other genotypes (psittacine and pigeon) were less common and were linked to exposure to caged birds or pigeons. The SNP-genotype Mat116 is rare, but predominated in this study. The use of SNP-based PCR provided a better understanding of the C. psittaci transmission from birds to humans compared to ompA analysis. In Sweden, human psittacosis appears mainly to be transmitted from garden birds during bird feeding in the winter season. (c) 2023 The Author(s). Published by Elsevier Masson SAS on behalf of Institut Pasteur. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

引用

页数：8