Lineage-tracing hematopoietic stem cell origins in vivo to efficiently make human HLF plus HOXA plus hematopoietic from pluripotent stem cells

被引：12

作者：

Fowler, Jonas L. ^{[1
,2
,11
]}

Zheng, Sherry Li ^{[1
,2
]}

Nguyen, Alana ^{[1
,2
,12
]}

Chen, Angela ^{[1
,2
,3
,13
]}

Xiong, Xiaochen ^{[1
,2
,14
]}

Chai, Timothy ^{[1
,2
]}

Chen, Julie Y. ^{[1
,2
]}

Karigane, Daiki ^{[1
,3
]}

Banuelos, Allison M. ^{[1
,4
]}

Niizuma, Kouta ^{[1
,5
]}

Kayamori, Kensuke ^{[1
,3
]}

Nishimura, Toshinobu ^{[1
,5
,15
]}

Cromer, M. Kyle ^{[6
]}

Gonzalez-Perez, David ^{[7
]}

Mason, Charlotte ^{[7
]}

Liu, Daniel Dan ^{[1
,4
]}

Yilmaz, Leyla ^{[1
,4
]}

Miquerol, Lucile ^{[8
]}

Porteus, Matthew H. ^{[1
,9
]}

Luca, Vincent C. ^{[7
]}

Majeti, Ravindra ^{[1
,3
]}

Nakauchi, Hiromitsu ^{[1
]}

Red-Horse, Kristy ^{[1
,10
]}

Weissman, Irving L. ^{[1
,4
]}

Ang, Lay Teng ^{[1
]}

Loh, Kyle M. ^{[1
,2
]}

机构：

[1] Stanford Univ, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Dev Biol, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Med, Div Hematol, Stanford, CA 94305 USA

[4] Stanford Univ, Dept Pathol, Stanford, CA 94305 USA

[5] Stanford Univ, Dept Genet, Stanford, CA 94305 USA

[6] Univ Calif San Francisco, Dept Surg, San Francisco, CA 94143 USA

[7] H Lee Moffitt Canc Ctr & Res Inst, Dept Drug Discovery, Tampa, FL 33612 USA

[8] Aix Marseille Univ, IBDM, CNRS UMR 7288, F-13288 Marseille, France

[9] Stanford Univ, Dept Pediat, Stanford, CA 94305 USA

[10] Stanford Univ, Howard Hughes Med Inst, Dept Biol, Stanford, CA 94305 USA

[11] Walking Fish Therapeut, South San Francisco, CA USA

[12] Univ Calif Davis, Sch Vet Med, Davis, CA 95616 USA

[13] Orca Bio, Menlo Pk, CA 94025 USA

[14] Stanford Univ, Dept Biol, Stanford, CA 94305 USA

[15] Century Therapeut, Philadelphia, PA 19104 USA

来源：

DEVELOPMENTAL CELL | 2024年 / 59卷 / 09期

关键词：

DEFINITIVE HEMATOPOIESIS; HAEMOGENIC ENDOTHELIUM; PROGENITOR CELLS; CLONAL ANALYSIS; YOLK-SAC; HEMOGENIC ENDOTHELIUM; AORTIC ENDOTHELIUM; MESODERM FORMATION; PRIMITIVE STREAK; RETINOIC ACID;

D O I：

10.1016/j.devcel.2024.03.003

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The developmental origin of blood -forming hematopoietic stem cells (HSCs) is a longstanding question. Here, our non-invasive genetic lineage tracing in mouse embryos pinpoints that artery endothelial generate HSCs. Arteries are transiently competent to generate HSCs for 2.5 days (similar to E8.5-E11) but subsequently cease, delimiting a narrow time frame for HSC formation in vivo . Guided by the arterial origins blood, we efficiently and rapidly differentiate human pluripotent stem cells (hPSCs) into posterior primitive streak, lateral mesoderm, artery endothelium, hemogenic endothelium, and >90% pure hematopoietic genitors within 10 days. hPSC-derived hematopoietic progenitors generate T, B, NK, erythroid, and myeloid cells in vitro and, critically, express hallmark HSC transcription factors HLF and HOXA5-HOXA10 , which previously challenging to upregulate. We differentiated hPSCs into highly enriched HLF + HOXA + hematopoietic progenitors with near-stoichiometric efficiency by blocking formation of unwanted lineages at each ferentiation step. hPSC-derived HLF + HOXA + hematopoietic progenitors could avail both basic research cellular therapies.

引用

页码：1110 / 1131.e22

页数：45

共 48 条

[41] Human proT-cells generated in vitro facilitate hematopoietic stem cell-derived T-lymphopoiesis in vivo and restore thymic architecture [J].

Awong, Geneve ;

Singh, Jastaranpreet ;

Mohtashami, Mahmood ;

Malm, Maria ;

La Motte-Mohs, Ross N. ;

Benveniste, Patricia M. ;

Serra, Pablo ;

Herer, Elaine ;

van den Brink, Marcel R. ;

Zuniga-Pfluecker, Juan Carlos .

BLOOD, 2013, 122 (26) :4210-4219

[42] Expression of RUNX1-JAK2 in Human Induced Pluripotent Stem Cell-Derived Hematopoietic Cells Activates the JAK-STAT and MYC Pathways [J].

Fortschegger, Klaus ;

Husa, Anna-Maria ;

Schinnerl, Dagmar ;

Nebral, Karin ;

Strehl, Sabine .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2021, 22 (14)

[43] Enhanced Ex Vivo Generation of Erythroid Cells from Human Induced Pluripotent Stem Cells in a Simplified Cell Culture System with Low Cytokine Support [J].

Bernecker, Claudia ;

Ackermann, Mania ;

Lachmann, Nico ;

Rohrhofer, Lisa ;

Zaehres, Holm ;

Arauzo-Bravo, Marcos J. ;

van den Akker, Emile ;

Schlenke, Peter ;

Dorn, Isabel .

STEM CELLS AND DEVELOPMENT, 2019, 28 (23) :1540-1551

[44] Polybenzyl Glutamate Biocompatible Scaffold Promotes the Efficiency of Retinal Differentiation toward Retinal Ganglion Cell Lineage from Human-Induced Pluripotent Stem Cells [J].

Chen, Ta-Ching ;

She, Pin-Yi ;

Chen, Dong Feng ;

Lu, Jui-Hsien ;

Yang, Chang-Hao ;

Huang, Ding-Siang ;

Chen, Pao-Yang ;

Lu, Chen-Yu ;

Cho, Kin-Sang ;

Chen, Hsin-Fu ;

Su, Wei-Fang .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2019, 20 (01)

[45] A novel lentiviral vector targets gene transfer into human hematopoietic stem cells in marrow from patients with bone marrow failure syndrome and in vivo in humanized mice [J].

Frecha, Cecilia ;

Costa, Caroline ;

Negre, Didier ;

Amirache, Fouzia ;

Trono, Didier ;

Rio, Paula ;

Bueren, Juan ;

Cosset, Francois-Loic ;

Verhoeyen, Els .

BLOOD, 2012, 119 (05) :1139-1150

[46] High-Efficiency Transduction of Primary Human Hematopoietic Stem Cells and Erythroid Lineage-Restricted Expression by Optimized AAV6 Serotype Vectors In Vitro and in a Murine Xenograft Model In Vivo [J].

Song, Liujiang ;

Li, Xiaomiao ;

Jayandharan, Giridhara R. ;

Wang, Yuan ;

Aslanidi, George V. ;

Ling, Chen ;

Zhong, Li ;

Gao, Guangping ;

Yoder, Mervin C. ;

Ling, Changquan ;

Tan, Mengqun ;

Srivastava, Arun .

PLOS ONE, 2013, 8 (03)

[47] Phorbol ester induced ex vivo expansion of rigorously-defined phenotypic but not functional human cord blood hematopoietic stem cells: a cautionary tale demonstrating that phenotype does not always recapitulate stem cell function [J].

Chen, Yandan ;

Yao, Chunxu ;

Teng, Yincheng ;

Jiang, Rongzhen ;

Huang, Xinxin ;

Liu, Sheng ;

Wan, Jun ;

Broxmeyer, Hal E. ;

Guo, Bin .

LEUKEMIA, 2019, 33 (12) :2962-2966

[48] Development of Human in vitro Brain-blood Barrier Model from Induced Pluripotent Stem Cell-derived Endothelial Cells to Predict the in vivo Permeability of Drugs [J].

Li, Yuan ;

Sun, Xueying ;

Liu, Houfu ;

Huang, Liang ;

Meng, Guofeng ;

Ding, Yu ;

Su, Wenji ;

Lu, Jiaqi ;

Gong, Sophie ;

Terstappen, Georg C. ;

Zhang, Ru ;

Zhang, Wandong .

NEUROSCIENCE BULLETIN, 2019, 35 (06) :996-1010

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