Evaluation of Dry Ice for Short-Term Storage and Transportation of Frozen Boar Semen

被引：0

作者：

He, Mengqian ^{[1
,2
,3
]}

Sun, Lingwei ^{[1
,2
,3
]}

Xu, Jiehuan ^{[1
,2
,3
]}

Wu, Caifeng ^{[1
,2
,3
]}

Zhang, Shushan ^{[1
,2
,3
]}

Gao, Jun ^{[1
,2
]}

Zhang, Defu ^{[1
,2
,3
]}

Gan, Yeqing ^{[4
]}

Bian, Yi ^{[4
]}

Wei, Jinliang ^{[4
]}

Zhang, Weijian ^{[5
]}

Zhang, Wengang ^{[5
]}

Han, Xuejun ^{[3
]}

Dai, Jianjun ^{[1
,2
,3
]}

机构：

[1] Shanghai Acad Agr Sci, Inst Anim Husb & Vet Sci, Shanghai Municipal Key Lab Agrigenet & Breeding, Shanghai 201106, Peoples R China

[2] Minist Agr & Rural Affairs, Key Lab Livestock & Poultry Resources Pig Evaluat, Shanghai 201106, Peoples R China

[3] Shanghai Engn Res Ctr Breeding Pig, Shanghai 201106, Peoples R China

[4] Shanghai Jiading Municipal Ctr Dis Control & Preve, Shanghai 201899, Peoples R China

[5] Shanghai Municipal Ctr Dis Control & Prevent, Shanghai 200051, Peoples R China

来源：

ANIMALS | 2024年 / 14卷 / 10期

关键词：

semen cryopreservation; dry ice; semen transportation; boar; SPERM; CRYOPRESERVATION; SPERMATOZOA; CORRELATE; QUALITY;

D O I：

10.3390/ani14101422

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

To address the safety problems posed by the transportation of boar semen using LN, this study was conducted on the short-term storage of frozen boar semen in dry ice (-79 degrees C). Boar semen frozen in LN was transferred to dry ice, kept for 1 day, 3 days, 5 days, 7 days, or 8 days, and then moved back to LN. The quality of frozen semen stored in LN or dry ice was determined to evaluate the feasibility of short-distance transportation with dry ice. The results showed that 60 degrees C for 8 s was the best condition for thawing frozen semen stored in dry ice. No significant differences in spermatozoa motility, plasma membrane integrity, or acrosome integrity were observed in semen after short-term storage in dry ice compared to LN (p > 0.05). There were no significant changes in antioxidant properties between storage groups either (p > 0.05). In conclusion, dry ice could be used as a cold source for the short-term transportation of frozen boar semen for at least 7 days, without affecting sperm motility, morphological integrity, or antioxidant indices.

引用

页数：12

共 50 条

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