Distinct tomato yellow leaf curl Chuxiong virus isolated from whiteflies and plants in China and its symptom determinant and suppressor of post-transcriptional gene silencing

被引：0

作者：

Xie, Yan ^{[1
]}

Liu, Xianan ^{[1
]}

Luo, Chaohu ^{[1
]}

Hu, Qianqian ^{[1
]}

Che, Xuan ^{[1
]}

Zhao, Liling ^{[2
]}

Zhao, Min ^{[1
]}

Wu, Liqi ^{[1
]}

Ding, Ming ^{[2
]}

机构：

[1] Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol & Breeding,Key Lab Biol Cr, Minist Agr,Key Lab Mol Biol Crop Pathogens & Insec, Hangzhou 310058, Peoples R China

[2] Yunnan Acad Agr Sci, Inst Biotechnol & Germplasm Resources, Key Lab Agr Biotechnol Yunnan Prov, Kunming 650223, Peoples R China

来源：

VIROLOGY | 2024年 / 594卷

基金：

中国国家自然科学基金;

关键词：

Begomovirus; Tomato yellow leaf curl Chuxiong virus; Whitefly; Molecular identity; Symptom determinant; Post-transcriptional gene silencing; GEMINIVIRUS DISEASE COMPLEXES; DIFFERENTIAL ROLES; BEGOMOVIRUS; DNA; PROTEIN; C4; EVOLUTION;

D O I：

10.1016/j.virol.2024.110040

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A begomovirus isolated from whiteflies (Bemisia tabaci) and tomato, sweet potato in China was found to be representative of a distinct begomovirus species, for which the name tomato yellow leaf curl Chuxiong virus (TYLCCxV) is proposed. The results of genomic identification and sequence comparison showed that TYLCCxV shares the highest complete nucleotide sequence identity (88.3%) with croton yellow vein mosaic virus (CroYVMV), and may have originated from the recombination between synedrella leaf curl virus (SyLCV) and squash leaf curl Yunnan virus (SLCuYV). Agrobacterium-mediated inoculation showed that TYLCCxV is highly infectious for a range of plant species, producing upward leaf curling, leaf crumpling, chlorosis, distortion, and stunt symptoms in Solanum lycopersicum plants. The results of Southern blot indicated that TYLCCxV is capable of efficiently replicating two heterologous betasatellites. The inoculation of PVX::C4 on Nicotiana benthamiana induced upward leaf curling and stem elongation symptoms, suggesting that TYLCCxV C4 functions as a symptom determinant. TYLCCxV V2 is an important virulence factor that induces downward leaf curling symptoms, elicits systemic necrosis, and suppresses local and systemic GFP silencing in co-agroinfiltrated N. benthamiana and transgenic 16c plants. Considering the multifunctional virulence proteins V2 and C4, the possibility of TYLCCxV causing devastating epidemics on tomato in China is discussed.

引用

页数：11

共 15 条

[1] The novel C5 protein from tomato yellow leaf curl virus is a virulence factor and suppressor of gene silencing [J].

Zhao, Siwen ;

Gong, Pan ;

Ren, Yanxiang ;

Liu, Hui ;

Li, Hao ;

Li, Fangfang ;

Zhou, Xueping .

STRESS BIOLOGY, 2022, 2 (01)

[2] V2 protein encoded by Tomato yellow leaf curl China virus is an RNA silencing suppressor [J].

Zhang, Jie ;

Dong, Jingyun ;

Xu, Yi ;

Wu, Jianxiang .

VIRUS RESEARCH, 2012, 163 (01) :51-58

[3] Detoxification activity and energy cost is attenuated in whiteflies feeding on Tomato yellow leaf curl China virus-infected tobacco plants [J].

Luan, J. -B. ;

Wang, Y. -L. ;

Wang, J. ;

Wang, X. -W. ;

Liu, S. -S. .

INSECT MOLECULAR BIOLOGY, 2013, 22 (05) :597-607

[4] Molecular characterization of Tomato yellow leaf curl China virus and its satellite DNA isolated from tobacco [J].

XIE Yan ZHOU Xueping LI Guixin Institute of Biotechnology Zhejiang University Hangzhou China Correspondence should be addressed to Zhou Xueping email zzhouzjueducn .

ChineseScienceBulletin, 2003, (08) :766-770

[5] Molecular characterization of Tomato yellow leaf curl China virus and its satellite DNA isolated from tobacco [J].

Xie, Y ;

Zhou, XP ;

Li, GX .

CHINESE SCIENCE BULLETIN, 2003, 48 (08) :766-770

[6] C4, the Pathogenic Determinant of Tomato Leaf Curl Guangdong Virus, May Suppress Post-transcriptional Gene Silencing by Interacting With BAM1 Protein [J].

Li, Zhenggang ;

Du, Zhenguo ;

Tang, Yafei ;

She, Xiaoman ;

Wang, Xiaomei ;

Zhu, Yanhua ;

Yu, Lin ;

Lan, Guobing ;

He, Zifu .

FRONTIERS IN MICROBIOLOGY, 2020, 11

[7] V2 of tomato yellow leaf curl virus can suppress methylation-mediated transcriptional gene silencing in plants [J].

Wang, Bi ;

Li, Fangfang ;

Huang, Changjun ;

Yang, Xiuling ;

Qian, Yajuan ;

Xie, Yan ;

Zhou, Xueping .

JOURNAL OF GENERAL VIROLOGY, 2014, 95 :225-230

[8] Cucurbit yellow stunting disorder virus p25 is a suppressor of post-transcriptional gene silencing [J].

Kataya, Amr R. A. ;

Suliman, Mohamed N. S. ;

Kalantidis, Kriton ;

Livieratos, Ioannis C. .

VIRUS RESEARCH, 2009, 145 (01) :48-53

[9] Ageratum yellow vein virus isolated from tomato plants with leaf curl on Ishigaki Island, Okinawa, Japan [J].

Andou, Tsunaki ;

Yamaguchi, Ayako ;

Kawano, Shinji ;

Kawabe, Kunimasa ;

Ueda, Shigenori ;

Onuki, Masatoshi .

JOURNAL OF GENERAL PLANT PATHOLOGY, 2010, 76 (04) :287-291

[10] Post-transcriptional and Epigenetic Arms of RNA Silencing: A Defense Machinery of Naturally Tolerant Tomato Plant Against Tomato Leaf Curl New Delhi Virus [J].

Sahu, Pranav Pankaj ;

Sharma, Namisha ;

Puranik, Swati ;

Prasad, Manoj .

PLANT MOLECULAR BIOLOGY REPORTER, 2014, 32 (05) :1015-1029

← 1 2 →