A novel polysaccharide of Undaria pinnatifida: Structural characterization, carboxymethylation and hypoglycemic activity in vivo

被引：16

作者：

Li, Can ^{[1
]}

Liu, Ying ^{[1
]}

Zhang, Xin ^{[1
]}

Liu, Huiping ^{[1
]}

Zhang, Huihui ^{[1
]}

Zhang, Xiaojing ^{[1
]}

Zhang, Xiaowei ^{[1
]}

Ma, Xiaoxiao ^{[1
]}

Wang, Bing ^{[1
]}

Xue, Tianrui ^{[1
]}

Lv, Binfei ^{[1
]}

Zhang, Mingran ^{[1
]}

机构：

[1] Tianjin Univ Sci & Technol, Coll Food Sci & Engn, State Key Lab Food Nutr & Safety, Minist Educ China, Tianjin 300457, Peoples R China

来源：

FOOD BIOSCIENCE | 2024年 / 60卷

关键词：

Carboxymethylation; Hypoglycemic; Polysaccharide; Preliminary structural characterization; Undaria pinnatifida; PHYSICOCHEMICAL PROPERTIES; DEGRADED POLYSACCHARIDES; AURICULARIA-AURICULA; VITRO ANTIOXIDANT; LACHNUM; PURIFICATION; DERIVATIVES; EXTRACTION; JAPONICA; FUCOIDAN;

D O I：

10.1016/j.fbio.2024.104479

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A new polysaccharide (UPP) was isolated and purified from Undaria pinnatifida with a low molecular weight of 8.34 kDa. The UPP was modified to obtain carboxymethylated polysaccharide (CM-UPP). UPP and CM-UPP were characterized by FT-IR, Ion chromatography, Congo red, DSC, SEM and NMR, and their hypoglycemic activity in vivo was investigated using the STZ-induced diabetes mice model. UPP mainly contained Ara, Gal, Glc and Man at a molar ratio of 0.18: 0.13: 2.06: 1, and CM-UPP added two alduronic acids including GalA and GlcA. CM-UPP had been prepared successfully and the carboxymethylation reaction might occur partially at the C-2 and C-4 position. There were no triple helix structures in both UPP and CM-UPP. In vivo hypoglycemic activity studies showed that both UPP and CM-UPP could alleviate symptoms of high blood glucose, improve blood lipid level, oxidative stress state and liver glycolytic enzyme activity in diabetic mice. Under the same dose, CM-UPP showed a more significant effect than UPP. These results indicated that carboxymethylation was an effective way to enhance the hypoglycemic activitiy of polysaccharide. UPP and CM-UPP could be potential therapeutic agents of T2DM.

引用

页数：12

共 54 条

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