DNA barcoding for identification of forensically important synanthropic flesh flies (Diptera: Sarcophagidae) from Eastern India

被引：2
作者：
Kar, Oishik [1 ]
Ghosh, Debjani [1 ]
Mukherjee, Arka [1 ]
Mukherjee, Koustav [1 ]
Pramanik, Debdeep [1 ]
Sarkar, Saikat [1 ]
Naskar, Atanu [1 ]
Banerjee, Dhriti [1 ]
机构：
[1] Zool Survey India, Diptera Sect, M Block, Kolkata 700053, India
来源：
ORIENTAL INSECTS | 2024年 / 58卷 / 04期
关键词：
Forensic entomology; synanthropy; post-mortem interval; COI gene; molecular identification; SPECIES DIPTERA; POSTMORTEM INTERVAL; FLY; CALLIPHORIDAE; BIODIVERSITY; CALYPTRATAE; CADAVERS; VECTORS; BOGOTA; CHINA;
D O I：
10.1080/00305316.2024.2356682
中图分类号：
Q96 [昆虫学];
学科分类号：
摘要：
Sarcophagidae is a significant Diptera family because several of its species are among the first to visit a corpse, and it helps in minPMI estimation. Sarcophagidae species identification for a layman is difficult due to identical morphological appearance and if the fly is a female. Also, fly specimens get damaged due to improper storage of forensic specimens collected from crime scenes. Thus, in this study, we aimed to check the efficiency of cytochrome oxidase 1 (COI) for identifying eleven sarcophagid flies species. Flesh flies were collected from various biogeographic regions of West Bengal. COI barcodes successfully differentiated between species with a very significant K2P interspecific genetic divergence, which ranged between 3.12% and 20.11%, and intraspecific genetic divergences ranged from 0% to 1%. The subgenera Uroxanthisca and Sinonipponia are used in the phylogenetic analysis for the first time in the oriental context. Phylogenetic analysis was performed using a Neighbour Joining (NJ) tree constructed using Kimura two-parameter (K2P) in MEGA X software. Thus, COI barcodes proved to be quite effective as an alternate method of identifying flesh fly species, and future studies should include additional markers for further phylogenetic investigations of the Sarcophagidae family.
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页码：658 / 678
页数：21
相关论文
共 77 条
[71]  
Wells JD, 2001, J FORENSIC SCI, V46, P1098
[72]   DNA-based identification of forensically important Chrysomyinae (Diptera: Calliphoridae) [J].
Wells, JD ;
Sperling, FAH .
FORENSIC SCIENCE INTERNATIONAL, 2001, 120 (1-2) :110-115
[73]  
White RS., 1940, FAUNA BRIT INDIA DIP, P6
[74]  
Yepes-Gaurisas D, 2013, REV BIOL TROP, V61, P1275
[75]   Application of DNA barcoding for identifying forensically relevant Diptera from northern Thailand [J].
Zajac, Barbara Karolina ;
Sontigun, Narin ;
Wannasan, Anchalee ;
Verhoff, Marcel A. ;
Sukontason, Kabkaew ;
Amendt, Jens ;
Zehner, Richard .
PARASITOLOGY RESEARCH, 2016, 115 (06) :2307-2320
[76]   Genetic identification of forensically important flesh flies (Diptera: Sarcophagidae) [J].
Zehner, R ;
Amendt, J ;
Schütt, S ;
Sauer, J ;
Krettek, R ;
Povolny, D .
INTERNATIONAL JOURNAL OF LEGAL MEDICINE, 2004, 118 (04) :245-247
[77]   Animal biodiversity: An outline of higher-level classification and taxonomic richness [J].
Zhang, Zhi-Qiang ;
Hooper, John N. A. ;
Van Soest, Rob W. M. ;
Pisera, Andrzej ;
Crowther, Andrea L. ;
Tyler, Seth ;
Schilling, Stephen ;
Eschmeyer, William N. ;
Fong, Jon D. ;
Blackburn, David C. ;
Wake, David B. ;
Wilson, D. E. ;
Reeder, DeeAnn M. ;
Fritz, Uwe ;
Hodda, Mike ;
Guidetti, Roberto ;
Bertolani, Roberto ;
Mayer, Georg ;
Oliveira, Ivo de Sena ;
Zhang, Zhi-Qiang ;
Adrain, Jonathan M. ;
Bamber, Roger N. ;
Kury, Adriano B. ;
Prendini, Lorenzo ;
Harvey, Mark S. ;
Beaulieu, Frederic ;
Dowling, Ashley P. G. ;
Klompen, Hans ;
de Moraes, Gilberto J. ;
Walter, David Evans ;
Zhang, Zhi-Qiang ;
Fan, Qing-Hai ;
Pesic, Vladimir ;
Smit, Harry ;
Bochkov, Andre V. ;
Khaustov, A. A. ;
Baker, Anne ;
Wohltmann, Andreas ;
Wen, Tinghuan ;
Amrine, James W. ;
Beron, P. ;
Lin, Jianzhen ;
Gabrys, Grzegorz ;
Husband, Robert ;
Bolton, Samuel ;
Uusitalo, M. ;
Zhang, Zhi-Qiang ;
Schatz, Heinrich ;
Behan-Pelletier, Valerie M. ;
OConnor, Barry M. .
ZOOTAXA, 2011, (3148) :7-237
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