Evaluation of protective immunity induced by a DNA vaccine encoding SAG2 and SRS2 against Toxoplasma gondii infection in mice

被引：1

作者：

Guo, Xu-Dong ^{[1
,2
]}

Zhou, Chun-Xue ^{[2
]}

Cui, Lin-Lin ^{[1
]}

Qiu, Hui-Jie ^{[2
]}

Wang, Yong-Liang ^{[2
]}

Fu, Ming ^{[2
]}

Liu, Dai-Ang ^{[2
]}

Han, Bing ^{[2
]}

Zhou, Huai-Yu ^{[2
]}

Zhou, Dong-Hui ^{[1
]}

机构：

[1] Fujian Agr & Forestry Univ, Coll Anim Sci, Key Lab Fujian Taiwan Anim Pathogen Biol, Fuzhou 350002, Fujian, Peoples R China

[2] Shandong Univ, Cheeloo Coll Med, Sch Basic Med Sci, Dept Pathogen Biol, Jinan 250012, Shandong, Peoples R China

来源：

ACTA TROPICA | 2024年 / 257卷

关键词：

Toxoplasma gondii; DNA vaccine; TgSAG2; TgSRS2; IMMUNIZATION;

D O I：

10.1016/j.actatropica.2024.107302

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Toxoplasma gondii is an important protozoan pathogen, which can cause severe diseases in the newborns and immunocompromised individuals. Developing an effective vaccine against Toxoplasma infection is a critically important global health priority. Immunofluorescence staining analysis revealed that TgSAG2 and TgSRS2 are membrane associated and displayed on the surface of the parasite. Immunizations with pBud-SAG2, pBud-SRS2 and pBud-SAG2-SRS2 DNA vaccines significantly increased the production of specific IgG antibodies. Immunization with pBud-SAG2-SRS2 elicited cellular immune response with higher concentrations of IFN- gamma and IL-4 compared to the control group. Antigen-specific lymphocyte proliferations in the pBud-SRS2 and pBud-SAG2SRS2 groups were significantly higher compared to that in the control group. Furthermore, 30 % of mice immunized with pBud-SAG2-SRS2 survived after the challenge infection with virulent T. gondii RH tachyzoites. This study revealed that immunization with pBud-SAG2-SRS2 induced potent immune responses, and has the potential as a promising vaccine candidate for the control of T. gondii infection.

引用

页数：7

共 26 条

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