Value of ultra-high b-value diffusion-weighted imaging for the evaluation of renal ischemia-reperfusion injury

To explore the feasibility of ultra-high b-value diffusion-weighted imaging (ubDWI) in assessment of renal IRI. Thirty-five rabbits were randomized into a control group (n = 7) and a renal IRI group (n = 28). The rabbits in the renal IRI group underwent left renal artery clamping for 60 min. Rabbits underwent axial ubDWI before and at 1, 12, 24, and 48 h after IRI. Apparent diffusion coefficient (ADCst) were calculated from ubDWI with two bvalues (b = 0, 1000 s/mm2). Triexponential fits were applied to calculate the pure diffusion coefficients (D), perfusion-related diffusion coefficient (D*), and ultra-high ADC (ADC(uh)). The interobserver reproducibility were evaluated. The repeated measurement analysis of variance and Spearman correlation analysis was used for statistical analysis. The ADC(st), D, and ADC(uh )values showed good reproducibility. The ADC(st), D, and D* values of renal Cortex (CO) and outer medulla (OM) significantly decreased after IRI (all P < 0.05). The ADCuh values significantly increased from pre-IRI to 1 h after IRI (P < 0.05) and significantly declined at 24 h and 48 h after IRI (all P < 0.05). ADCuh was strongly positively correlated with AQP-1 in the renal CO and OM (rho = 0.643, P < 0.001; rho = 0.662, P < 0.001, respectively). ubDWI can be used to non-invasively evaluate early renal IRI, ADC(uh )may be adopted to reflect AQP-1 expression.