Coexistence of a novel chromosomal integrative and mobilizable element Tn 7548 with two bla KPC-2-carrying plasmids in a multidrug-resistant Aeromonas hydrophila strain K522 from China

被引：1

作者：

Jing, Ying ^{[1
]}

Yu, Sufei ^{[2
]}

Li, Zhaolun ^{[1
]}

Ma, Jie ^{[1
]}

Wang, Luwei ^{[1
]}

Yu, Lianhua ^{[3
]}

Song, Zhiwei ^{[3
]}

Chen, Huimin ^{[3
]}

Wu, Zhenghai ^{[4
]}

Luo, Xinhua ^{[3
]}

机构：

[1] Capital Med Univ, Beijing Childrens Hosp, Dept Clin Lab Ctr, Natl Ctr Childrens Hlth, Beijing, Peoples R China

[2] Wenzhou Med Univ, Taizhou Hosp Zhejiang Prov, Linhai, Peoples R China

[3] Taizhou Municipal Hosp, Dept Clin Lab Med, Taizhou, Peoples R China

[4] Tradit Chinese Med Hosp Huangyan, Dept Clin Lab Med, Taizhou, Peoples R China

来源：

JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE | 2024年 / 37卷

关键词：

Aeromonas hydrophila; elements; Novel mobile genetic elements; blaKPC-2; Tn 7412-related integrative and mobilizable; METALLO-BETA-LACTAMASE; SURVEILLANCE; DATABASE; CAVIAE; GENE;

D O I：

10.1016/j.jgar.2024.03.006

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Objectives: Herein, we detected one multidrug-resistant Aeromonas hydrophila strain K522 co -carrying two bla KPC -2 genes together with a novel chromosomal integrative and mobilizable element (IME) Tn 7548 from China. To reveal the genetic characteristics of the novel reservoir of bla KPC-2 and IME in Aeromonas , a detailed genomic characterization of K522 was performed, and a phylogenetic analysis of Tn 7412 -related IMEs was carried out. Methods: Carbapenemases were detected by using the immunocolloidal gold technique and antimicrobial susceptibility was tested by using VITEK 2. The whole-genome sequences of K522 were analysed using phylogenetics, detailed dissection, and comparison. Results: Strain K522 carried a Tn 7412 -related chromosomal IME Tn 7548 and three resistance plasmids pK522-A-KPC, pK522-B-KPC, and pK522-MOX. A phylogenetic tree of 82 Tn 7412 -related IMEs was constructed, and five families of IMEs were divided. These IMEs shared four key backbone genes: int, repC , and hipAB , and carried various profiles of antimicrobial resistance genes (ARGs). pK522-A-KPC and pK522B-KPC carried bla KPC-2 and belonged to IncG and unclassified type plasmid, respectively. The bla KPC-2 regions of these two plasmids were the truncated version derived from Tn 6296 , resulting in the carbapenem resistance of K522. Conclusion: We first reported A. hydrophila harbouring a novel Tn 7412 -related IME Tn 7548 together with two bla KPC-2 carrying plasmids and a MDR plasmid. Three of these four mobile genetic elements (MGEs) discovered in A. hydrophila K522 were novel. The emergence of novel MGEs carrying ARGs indicated the rapid evolution of the resistance gene vectors in A. hydrophila under selection pressure and would contribute to the further dissemination of various ARGs in Aeromonas . (c) 2024 The Authors. Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy. This is an open access article under the CC BY -NC -ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )

引用

页码：157 / 164

页数：8