Determination of aztreonam/ceftazidime-avibactam synergism and proposal of a new methodology for the evaluation of susceptibility in vitro

被引：2

作者：

Collar, Gabriela da Silva ^{[1
,2
,4
]}

Moreira, Natalia Kehl ^{[1
,2
]}

Becker, Julia ^{[1
]}

Barth, Afonso Luis ^{[2
,3
]}

Caierao, Juliana ^{[1
,2
]}

机构：

[1] Univ Fed Rio Grande Do Sul, Fac Farm, Lab Pesquisa Bacteriol Clin LaBaC, Porto Alegre, Brazil

[2] Univ Fed Rio Grande Do Sul, Fac Farm, Programa Posgrad Ciencias Farmaceut, Porto Alegre, Brazil

[3] Hosp Clin Porto Alegre, Lab Pesquisa Resistencia Bacteriana LABRESIS, Porto Alegre, Brazil

[4] Univ Fed Rio Grande Do Sul, Fac Farm, Ave Ipiranga 2752, BR-90610000 Porto Alegre, RS, Brazil

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2024年 / 109卷 / 02期

关键词：

Aztreonam-avibactam; Ceftazidime-avibactam; Enterobacterales; Microelution ATM/CZA; P; aeruginosa; Synergism; CEFTAZIDIME-AVIBACTAM; AZTREONAM; SERINE;

D O I：

10.1016/j.diagmicrobio.2024.116236

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We proposed a new methodology, the microelution ATM/CZA (mATM/CZA), based on the antibiotic disc elution and the use of resazurin, for rapid (<4h) determination of in vitro susceptibility to aztreonam combined with ceftazidime-avibactam among Enterobacterales. The mATM/CZA presented excellent accuracy with 1.9 %, 98.1 % and 100 % of major error, specificity and sensitivity, respectively. Furthermore, we assessed synergism between aztreonam and ceftazidime-avibactam in Enterobacterales and Pseudomonas aeruginosa, which was observed in 37/55 Enterobacterales and 31/56 P. aeruginosa. As reference methodologies (checkerboard, time-kill curve) are not compatible with the routine of the clinical microbiology laboratories, mATM/CZA is an important alternative to evaluate susceptibility of the combination in a scenario where its clinical use is increasingly important.

引用

页数：8

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