A green EC/ECL dual-mode biosensing platform for detection of Vibrio parahaemolyticus

被引：4
作者：
Zhai, Tingting [1 ,2 ]
Zhang, Qingqing [2 ]
Yang, Fan [2 ]
Zhou, Huiqian [2 ]
Bai, Lipeng [2 ]
Hao, Tingting [2 ]
Guo, Zhiyong [2 ]
Chen, Zhongxiu [1 ]
机构：
[1] Zhejiang Gongshang Univ, Coll Food & Biol Engn, Hangzhou 310035, Peoples R China
[2] Ningbo Univ, Sch Mat Sci & Chem Engn, State Key Lab Managing Biot & Chem Threats Qual &, Ningbo 315211, Peoples R China
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2024年 / 415卷
基金：
中国国家自然科学基金;
关键词：
Vibrio parahaemolyticus; Electrochemistry; Electrochemiluminescence; Dual-mode sensing platform; Regeneration; RAPID DETECTION METHODS;
D O I：
10.1016/j.snb.2024.136010
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Here, a green EC/ECL dual-mode biosensing platform was designed for sensitive and accurate detection of Vibrio parahaemolyticus (VP). When VP was present, Apt on Fe3O4@SiO2-cDNA/Apt was specifically bound to VP and detached to obtain Fe3O4@SiO2-cDNA. Subsequently, aDNA on Zn-TCPP@AgNPs-aDNA was hybridized with cDNA on Fe3O4@SiO2-cDNA to obtain magnetic conjugates, which were quickly collected by magnetic force and then one-step assembled onto a magnetic glassy carbon (MGCE) electrode for EC/ECL dual-mode measurement. Under the optimal experimental conditions, the detection linear range of VP was 10 similar to 10(7) CFU/mL, and the detection limits (LOD) were as low as 7 CFU/mL (EC) and 3 CFU/mL (ECL), respectively, with good repeatability, storage stability and selectivity. In addition, Fe3O4@SiO2-cDNA/Apt and Zn-TCPP@AgNPs-aDNA could be regained by adding Apt into the magnetic conjugates recovered after EC/ECL test, thus the biosensing platform was regenerated with the signal still remaining up to 91.7% (EC) and 92.8% (ECL) after 10 cycles of recovery. With homogeneous recognition and good regeneration, this EC/ECL dual-mode sensing platform provides a green, simple and reliable pathway for the analysis of VP or other foodborne pathogens.
引用
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页数：9
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