Genetic engineering of a thermophilic acetogen, Moorella thermoacetica Y72, to enable acetoin production

被引:1
作者
Kato, Junya [1 ,2 ]
Fujii, Tatsuya [2 ]
Kato, Setsu [1 ]
Wada, Keisuke [3 ]
Watanabe, Masahiro [2 ]
Nakamichi, Yusuke [2 ]
Aoi, Yoshiteru [1 ]
Morita, Tomotake [2 ]
Murakami, Katsuji [2 ]
Nakashimada, Yutaka [1 ]
机构
[1] Hiroshima Univ, Grad Sch Integrated Sci Life, Higashihiroshima, Hiroshima, Japan
[2] Natl Inst Adv Ind Sci & Technol, Higashihiroshima, Hiroshima, Japan
[3] Natl Inst Adv Ind Sci & Technol, Tsukuba, Ibaraki, Japan
关键词
thermophilic acetogen; genetic engineering; C4; chemicals; acetoin; biomass; gaseous substrates; ETHANOL-PRODUCTION; TRANSFORMATION; FERMENTATION; 2,3-BUTANEDIOL; GROWTH;
D O I
10.3389/fbioe.2024.1398467
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Acetogens are among the key microorganisms involved in the bioproduction of commodity chemicals from diverse carbon resources, such as biomass and waste gas. Thermophilic acetogens are particularly attractive because fermentation at higher temperatures offers multiple advantages. However, the main target product is acetic acid. Therefore, it is necessary to reshape metabolism using genetic engineering to produce the desired chemicals with varied carbon lengths. Although such metabolic engineering has been hampered by the difficulty involved in genetic modification, a model thermophilic acetogen, M. thermoacetica ATCC 39073, is the case with a few successful cases of C2 and C3 compound production, other than acetate. This brief report attempts to expand the product spectrum to include C4 compounds by using strain Y72 of Moorella thermoacetica. Strain Y72 is a strain related to the type strain ATCC 39073 and has been reported to have a less stringent restriction-modification system, which could alleviate the cumbersome transformation process. A simplified procedure successfully introduced a key enzyme for acetoin (a C4 chemical) production, and the resulting strains produced acetoin from sugars and gaseous substrates. The culture profile revealed varied acetoin yields depending on the type of substrate and culture conditions, implying the need for further engineering in the future. Thus, the use of a user-friendly chassis could benefit the genetic engineering of M. thermoacetica.
引用
收藏
页数:7
相关论文
共 28 条
[1]   High-temperature fermentation: how can processes for ethanol production at high temperatures become superior to the traditional process using mesophilic yeast? [J].
Abdel-Banat, Babiker M. A. ;
Hoshida, Hisashi ;
Ano, Akihiko ;
Nonklang, Sanom ;
Akada, Rinji .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2010, 85 (04) :861-867
[2]   Regulation of the NADH pool and NADH/NADPH ratio redistributes acetoin and 2,3-butanediol proportion in Bacillus subtilis [J].
Bao, Teng ;
Zhang, Xian ;
Zhao, Xiaojing ;
Rao, Zhiming ;
Yang, Taowei ;
Yang, Shangtian .
BIOTECHNOLOGY JOURNAL, 2015, 10 (08) :1298-1306
[3]  
BAUCHOP T, 1960, J GEN MICROBIOL, V23, P457
[4]   Genetic and metabolic engineering challenges of C1-gas fermenting acetogenic chassis organisms [J].
Bourgade, Barbara ;
Minton, Nigel P. ;
Islam, M. Ahsanul .
FEMS MICROBIOLOGY REVIEWS, 2021, 45 (02)
[5]   Two propanediol utilization-like proteins of Moorella thermoacetica with phosphotransacetylase activity [J].
Breitkopf, Ronja ;
Uhlig, Ronny ;
Drenckhan, Tina ;
Fischer, Ralf-Joerg .
EXTREMOPHILES, 2016, 20 (05) :653-661
[6]   Acetogens: Biochemistry, Bioenergetics, Genetics, and Biotechnological Potential [J].
Debabov, V. G. .
MICROBIOLOGY, 2021, 90 (03) :273-297
[7]   Physiology of the thermophilic acetogen Moorella thermoacetica (vol 155, pg 422, 2004) [J].
Drake, HL ;
Daniel, SL .
RESEARCH IN MICROBIOLOGY, 2004, 155 (10) :868-883
[8]   Integrated bioprocess for conversion of gaseous substrates to liquids [J].
Hu, Peng ;
Chakraborty, Sagar ;
Kumar, Amit ;
Woolston, Benjamin ;
Liu, Hongjuan ;
Emerson, David ;
Stephanopoulos, Gregory .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2016, 113 (14) :3773-3778
[9]   Homolactic Acid Fermentation by the Genetically Engineered Thermophilic Homoacetogen Moorella thermoacetica ATCC 39073 [J].
Iwasaki, Yuki ;
Kita, Akihisa ;
Yoshida, Koichiro ;
Tajima, Takahisa ;
Yano, Shinichi ;
Shou, Tomohiro ;
Saito, Masahiro ;
Kato, Junichi ;
Murakami, Katsuji ;
Nakashimada, Yutaka .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2017, 83 (08)
[10]   Engineering of a functional thermostable kanamycin resistance marker for use in Moorella thermoacetica ATCC39073 [J].
Iwasaki, Yuki ;
Kita, Akihisa ;
Sakai, Shinsuke ;
Takaoka, Kazue ;
Yano, Shinichi ;
Tajima, Takahisa ;
Kato, Junichi ;
Nishio, Naomichi ;
Murakami, Katsuji ;
Nakashimada, Yutaka .
FEMS MICROBIOLOGY LETTERS, 2013, 343 (01) :8-12