Evaluation of SARS-CoV-2 interferon gamma release assay in BNT162b2 vaccinated healthcare workers

被引：1

作者：

Ramos, Angelica ^{[1
,2
,3
]}

Martins, Sandra ^{[1
,2
,3
]}

Marinho, Ana Sofia ^{[1
]}

Norton, Pedro ^{[2
,3
,4
]}

Cardoso, Maria Joao ^{[1
]}

Guimaraes, Joao Tiago ^{[1
,2
,3
,5
]}

机构：

[1] Ctr Hosp Univ Sao Joao, Serv Patol Clin, Porto, Portugal

[2] Univ Porto, EPIUnit, Inst Saude Publ, Porto, Portugal

[3] Univ Porto, Lab Invest Integrat & Translac Saude Populac ITR, Porto, Portugal

[4] Ctr Hosp Univ Sao Joao, Serv Saude Ocupac, Porto, Portugal

[5] Univ Porto, Fac Med, Dept Biomed, Porto, Portugal

来源：

PLOS ONE | 2024年 / 19卷 / 05期

关键词：

T-CELL RESPONSES; CYTOMEGALOVIRUS DISEASE; CMV INFECTION; B-CELLS; COVID-19; SEVERITY; IL-10; RISK;

D O I：

10.1371/journal.pone.0303244

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To predict protective immunity to SARS-CoV-2, cellular immunity seems to be more sensitive than humoral immunity. Through an Interferon-Gamma (IFN-gamma) Release Assay (IGRA), we show that, despite a marked decrease in total antibodies, 94.3% of 123 healthcare workers have a positive cellular response 6 months after inoculation with the 2nd dose of BNT162b2 vaccine. Despite the qualitative relationship found, we did not observe a quantitative correlation between IFN-gamma and IgG levels against SARS-CoV-2. Using stimulated whole blood from a subset of participants, we confirmed the specific T-cell response to SARS-CoV-2 by dosing elevated levels of the IL-6, IL-10 and TNF-alpha. Through a 20-month follow-up, we found that none of the infected participants had severe COVID-19 and that the first positive cases were only 12 months after the 2nd dose inoculation. Future studies are needed to understand if IGRA-SARS-CoV-2 can be a powerful diagnostic tool to predict future COVID-19 severe disease, guiding vaccination policies.

引用

页数：13

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