Polyhydroxybutyrate (PHB) production from crude glycerol by genetic engineering of Rhodotorula glutinis

被引：15

作者：

Aristya, Ganies Riza ^{[1
,2
,3
]}

Lind, Yu-Ju ^{[4
]}

Chang, Jo-Shu ^{[5
,6
]}

Chang, Jui-Jen ^{[1
,7
,8
]}

Yen, Hong-Wei ^{[1
]}

机构：

[1] Tunghai Univ, Dept Chem & Mat Engn, Taichung, Taiwan

[2] Tunghai Univ, Dept Life Sci, Taichung, Taiwan

[3] Univ Gadjah Mada, Fac Biol, Yogyakarta, Indonesia

[4] Natl Chung Hsing Univ, Dept Life Sci, Taichung, Taiwan

[5] Natl Cheng Kung Univ, Dept Chem & Mat Engn, Taichung, Taiwan

[6] Tunghai Univ, Res Ctr Smart Sustainable Circular Econ, Taichung, Taiwan

[7] China Med Univ, China Med Univ Hosp, Dept Med Res, Taichung, Taiwan

[8] China Med Univ, Grad Inst Integrated Med, Taichung, Taiwan

来源：

BIORESOURCE TECHNOLOGY REPORTS | 2022年 / 18卷

关键词：

SACCHAROMYCES-CEREVISIAE; POLYHYDROXYALKANOATES; CULTIVATION; BIOSYNTHESIS; IRRADIATION; PRODUCERS; YEASTS; GROWTH; LIPIDS; WASTE;

D O I：

10.1016/j.biteb.2022.101048

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Polyhydroxybutyrate (PHB) is a member of the Polyhydroxyalkanoates (PHAs) family, which is generally produced through microbial biosynthesis. The synthetic biology tools are applied to transform PHB genes (PhaA, PhaB and PhaC) into an oleaginous yeast (Rhodotorula glutinis) genome. Western blot analysis confirms the expression of PhaA, PhaB and PhaC in the candidate transformants. The strain of R. glutinis #100-29 was selected to be the best PHB production candidate according to the shaker results. As compared to glucose, crude glycerol was known to be a suitable carbon source for R. glutinis cultivation. The PHB concentration, yield and content obtained in a 5-L fermenter using 30 g/L of crude glycerol were 2.87 g/L, 95.53 mg/g and 62% respectively. To the best of our knowledge, these should be the highest numbers obtained in yeast for PHB production, which indicates that strain #100-29 could be a potential PHB producer from crude glycerol.

引用

页数：9

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