Exploring the effect of protein secondary structure on the solid state and physical stability of protein-based amorphous solid dispersions

Amorphous solid dispersions (ASDs) using proteins as carriers have emerged as a promising strategy for stabilizing amorphous drug molecules. Proteins possess diverse three-dimensional structures that significantly influence their own properties and may also impact the properties of ASDs. We prepared beta-lactoglobulin (BLG) with different contents of beta-sheet and alpha-helical secondary structures by initially dissolving BLG in different mixed solvents, containing different ratios of water, methanol/ethanol, and acetic acid, followed by spray drying of the solutions. Our findings revealed that an increase in alpha-helical content resulted in a decrease in the glass transition temperature (Tg) of the protein. Subsequently, we utilized the corresponding mixed solvents to dissolve both BLG and the model drug celecoxib (CEL), allowing the preparation of ASDs containing either beta-sheet-rich or alpha-helix/ random coil-rich BLG. Using spray drying, we successfully developed BLG-based ASDs with drug loadings ranging from 10 wt% to 90 wt%. At drug loadings below 40 wt%, samples prepared using both methods exhibited single-phase ASDs. However, heterogeneous systems formed when the drug loading exceeded 40 wt%. At higher drug loadings, physical stability assessments demonstrated that the alpha-helix/random coil-rich BLG structure exerted a more pronounced stabilizing effect on the drug-rich phase compared to the beta-sheet-rich BLG. Overall, our results highlight the importance of considering protein secondary structure in the design of ASDs.