Development of pH-Sensitive Film for Detection of Implant Infection via Ultrasound Luminescent Chemical Imaging

A new hybrid ultrasound luminescent chemical imaging technique is described along with a pH sensor to image chemical concentrations at the surface of implanted medical devices. The purpose is to detect and study local biochemistry during infection. The sensor comprises a mechanoluminescent film (SrAl2O4:Eu, Dy microphosphors embedded in a biocompatible polymer film) and a pH indicator dye. A focused ultrasound beam generates green luminescence at the ultrasound focal point. By pulsing the ultrasound ON and OFF, the modulated luminescence can be distinguished from persistent luminescence, for high spatial resolution imaging. A red fluorescent dye and the pH indicator dye bromothymol blue are added to the coating to modulate the red-light transmittance via pH dependent absorbance. Acidosis is observed as an increase in red luminescence intensity in spectroscopy and imaging. The films are sensitive to biologically relevant changes in pH (6.0-8.0) and can be imaged through optically scattering media to mimic tissue. The images have a knife edge spatial resolution of approximate to 3 mm through optically scattering phantoms, limited by the focused ultrasound spot size. This novel technique may permit the elucidation of implant infection at the implant surface and can be further developed for the measurement of other relevant chemical species in the future. To detect bacterial biofilm induced acidosis on the surface of implanted medical devices, an ultrasound luminescent chemical imaging (ULCI) technique is developed along with a pH-sensitive device coating. A focused ultrasound beam excites luminescence from mechanoluminescent phosphors in the coating and pH-sensitive bromothymol blue indicator modulates the luminescence collected through tissue mimics to generate pH maps. image