Tracking epithelial-mesenchymal transition in breast cancer cells based on a multiplex electrochemical immunosensor

被引:5
|
作者
Wei, Xue [1 ]
Xiong, Hanzhi [1 ]
Zhou, Yunfan [1 ]
Chen, Xu [1 ]
Yang, Wensheng [1 ]
机构
[1] Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China
来源
BIOSENSORS & BIOELECTRONICS | 2024年 / 258卷
基金
中国国家自然科学基金;
关键词
Epithelial-mesenchymal transition; Multiple electrochemical immunoassay; Dynamic monitoring; E-cadherin; N-cadherin; N-CADHERIN EXPRESSION; PLATFORM; NANOCOMPOSITE; COMPOSITE; BIOSENSOR; CHITOSAN; MARKERS; GROWTH; EMT;
D O I
10.1016/j.bios.2024.116372
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Epithelial-mesenchymal transition (EMT) promotes tumor cell infiltration and metastasis. Tracking the progression of EMT could potentially indicate early cancer metastasis. A key characteristic of EMT is the dynamic alteration in the molecular levels of E-cadherin and N-cadherin. Traditional assays have limited sensitivity and multiplexing capabilities, relying heavily on cell lysis. Here, we developed a multiplex electrochemical biosensor to concurrently track the upregulation of N-cadherin expression and reduction of E-cadherin in breast cancer cells undergoing EMT. Small-sized gold nanoparticles (Au NPs) tagged with redox probes (thionin or amino ferrocene) and bound to two types of antibodies were used as distinguishable signal tags. These tags specifically recognized E-cadherin and N-cadherin proteins on the tumor cell surface without cross-reactivity. The diphenylalanine dipeptide (FF)/chitosan (CS)/Au NPs (FF-CS@Au) composites with high surface area and good biocompatibility were used as the sensing platforms for efficiently fixing cells and recording the dynamic changes in electrochemical signals of surface proteins. The electrochemical immunosensor allowed for simultaneous monitoring of E- and N-cadherins on breast cancer cell surfaces in a single run, enabling tracking of the EMT dynamic process for up to 60 h. Furthermore, the electrochemical detection results are consistent with Western blot analysis, confirming the reliability of the methodology. This present work provides an effective, rapid, and low-cost approach for tracking the EMT process, as well as valuable insights into early tumor metastasis.
引用
收藏
页数:8
相关论文
共 50 条
  • [1] Epithelial-Mesenchymal Transition and Breast Cancer
    Wu, Yanyuan
    Sarkissyan, Marianna
    Vadgama, Jaydutt V.
    JOURNAL OF CLINICAL MEDICINE, 2016, 5 (02)
  • [2] Tracking the intermediate stages of epithelial-mesenchymal transition in epithelial stem cells and cancer
    Jordan, Nicole Vincent
    Johnson, Gary L.
    Abell, Amy N.
    CELL CYCLE, 2011, 10 (17) : 2865 - 2873
  • [3] Sef Regulates Epithelial-Mesenchymal Transition in Breast Cancer Cells
    He, Qing
    Gong, Yan
    Gower, Lindsey
    Yang, Xuehui
    Friesel, Robert E.
    JOURNAL OF CELLULAR BIOCHEMISTRY, 2016, 117 (10) : 2346 - 2356
  • [4] Hispidulin modulates epithelial-mesenchymal transition in breast cancer cells
    Kim, Hyun A.
    Lee, Joomin
    ONCOLOGY LETTERS, 2021, 21 (02)
  • [5] Breast Cancer Stem Cells and the Epithelial-Mesenchymal Transition.
    Weinberg, R. A.
    CANCER RESEARCH, 2009, 69 (24) : 484S - 484S
  • [6] The Importance of the Epithelial-Mesenchymal Transition in Breast Cancer
    Sphyris, Nathalie
    Mani, Sendurai A.
    CURRENT BREAST CANCER REPORTS, 2009, 1 (04) : 229 - 237
  • [7] The importance of the epithelial-mesenchymal transition in breast cancer
    Nathalie Sphyris
    Sendurai A. Mani
    Current Breast Cancer Reports, 2009, 1 (4) : 229 - 237
  • [8] Abnormalities in miRNAome contribute to epithelial-mesenchymal transition of breast cancer cells
    Halytskiy, V.
    EUROPEAN JOURNAL OF CANCER, 2017, 72 : S24 - S24
  • [9] Role of fucosyltransferase IV in epithelial-mesenchymal transition in breast cancer cells
    Yang, X.
    Liu, S.
    Yan, Q.
    CELL DEATH & DISEASE, 2013, 4 : e735 - e735
  • [10] Tracking and characterization of partial and full epithelial-mesenchymal transition cells in a mouse model of metastatic breast cancer
    Luond, Fabiana
    Santacroce, Natascha
    Beisel, Christian
    Guerard, Laurent
    Burglin, Thomas R.
    Christofori, Gerhard
    Sugiyama, Nami
    STAR PROTOCOLS, 2022, 3 (02):