The Molecular Characterization of the Cyprinid herpesvirus 3 (CyHV-3) ORF24 Protein and its effect on the expression of immune genes (in vitro)

被引:0
作者
Wang, Jing [1 ]
Ji, Yan [1 ]
Zhou, Xuan [2 ]
Yu, Denghang [1 ]
Tan, Kianann [3 ]
Zhang, Chi [1 ]
机构
[1] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China
[2] Technol Ctr Wuhan Customs, Wuhan, Peoples R China
[3] Beibu Gulf Univ, Coll Marine Sci, Guangxi Key Lab Beibu Gulf Marine Biodivers Conser, Qinzhou 535011, Guangxi, Peoples R China
来源
ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH | 2024年 / 76卷 / 01期
基金
中国国家自然科学基金;
关键词
RECEPTORS; VACCINE; CARP; IFN; RECOGNITION; DISEASE; KHV;
D O I
10.46989/001c.94381
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Cyprinid herpesvirus 3 (CyHV-3), known as koi herpesvirus (KHV), is highly contagious and lethal. In this study, we aimed to characterize the ORF24-encoding protein of CyHV-3, investigate its sub-cellular localization, and determine its impact on the expression of immune factors through in vitro experiments. The results showed that the CyHV-3 ORF24 protein comprises 579 amino acids. Interestingly, multiple comparisons with homologous proteins from three carp herpesvirus origins showed no significant similarity. The fluorescence localization experiment showed that the green fluorescence signal, representing the protein pEGFP-ORF24, was primarily diffused in the cytoplasm. Notably, the overexpression of ORF24 effectively suppressed the expression of immune factors in both CCO (Channel catfish ovary) and FHM (Fathead minnow muscle cell line) cells. Bioinformatic analysis indicated that the CyHV-3 ORF24 gene exhibited significant differences from the corresponding genes in the other two carp herpesviruses. This suggests its unique functional role in the evolutionary context. Moreover, our findings demonstrated that overexpression of CyHV-3 ORF24 can effectively inhibit the expression of immune factors, underscoring its crucial role as a viral immune escape factor. These results provide further insights into the immune function of the CyHV-3 ORF24 protein and offer a theoretical foundation for developing new vaccines against CyHV-3 virus infections.
引用
收藏
页数:8
相关论文
共 29 条
  • [1] Type I interferon responses of common carp strains with different levels of resistance to koi herpesvirus disease during infection with CyHV-3 or SVCV
    Adamek, Mikolaj
    Matras, Marek
    Dawson, Andy
    Piackova, Veronika
    Gela, David
    Kocour, Martin
    Adamek, Jerzy
    Kaminski, Rafal
    Rakus, Krzysztof
    Bergmann, Sven M.
    Stachnik, Magdalena
    Reichert, Michal
    Steinhagen, Dieter
    [J]. FISH & SHELLFISH IMMUNOLOGY, 2019, 87 : 809 - 819
  • [2] Susceptibility of koi x crucian carp and koi x goldfish hybrids to koi herpesvirus (KHV) and the development of KHV disease (KHVD)
    Bergmann, S. M.
    Sadowski, J.
    Kielpinski, M.
    Bartlomiejczyk, M.
    Fichtner, D.
    Riebe, R.
    Lenk, M.
    Kempter, J.
    [J]. JOURNAL OF FISH DISEASES, 2010, 33 (03) : 267 - 272
  • [3] Fish Immunology. The modification and manipulation of the innate immune system: Brazilian studies
    Biller-Takahashi, Jaqueline D.
    Urbinati, Elisabeth C.
    [J]. ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS, 2014, 86 (03): : 1483 - 1495
  • [4] Identification of an essential virulence gene of cyprinid herpesvirus 3
    Boutier, Maxime
    Gao, Yuan
    Vancsok, Catherine
    Suarez, Nicolas M.
    Davison, Andrew J.
    Vanderplasschen, Alain
    [J]. ANTIVIRAL RESEARCH, 2017, 145 : 60 - 69
  • [5] Diagnostic validation of three test methods for detection of cyprinid herpesvirus 3 (CyHV-3)
    Clouthier, Sharon C.
    McClure, Carol
    Schroeder, Tamara
    Desai, Megan
    Hawley, Laura
    Khatkar, Sunita
    Lindsay, Melissa
    Lowe, Geoff
    Richard, Jon
    Anderson, Eric D.
    [J]. DISEASES OF AQUATIC ORGANISMS, 2017, 123 (02) : 101 - 122
  • [6] Epstein-Barr virus: Biology and clinical disease
    Damania, Blossom
    Kenney, Shannon C.
    Raab-Traub, Nancy
    [J]. CELL, 2022, 185 (20) : 3652 - 3670
  • [7] Immune gene expression in cyprinid herpesvirus-2 (CyHV-2)-sensitized peripheral blood leukocytes (PBLs) co-cultured with CyHV-2-infected goldfish fin cell line
    Das, Sweta
    Dharmaratnam, Arathi
    Ravi, Charan
    Kumar, Raj
    Swaminathan, Thangaraj Raja
    [J]. AQUACULTURE INTERNATIONAL, 2021, 29 (05) : 1925 - 1934
  • [8] Expression profile of microrna-145 in urothelial bladder cancer
    Dip, Nelson
    Reis, Sabrina T.
    Srougi, Miguel
    Dall'Oglio, Marcos F.
    Leite, Katia R. M.
    [J]. INTERNATIONAL BRAZ J UROL, 2013, 39 (01): : 95 - 101
  • [9] The gut microbiota prime systemic antiviral immunity via the cGAS-STING-IFN-I axis
    Erttmann, Saskia F.
    Swacha, Patrycja
    Aung, Kyaw Min
    Brindefalk, Bjorn
    Jiang, Hui
    Hartova, Anetta
    Uhlin, Bernt Eric
    Wai, Sun N.
    Gekara, Nelson O.
    [J]. IMMUNITY, 2022, 55 (05) : 847 - +
  • [10] CyHV-3: the third cyprinid herpesvirus
    Gotesman, Michael
    Kattlun, Julia
    Bergmann, Sven M.
    El-Matbouli, Mansour
    [J]. DISEASES OF AQUATIC ORGANISMS, 2013, 105 (02) : 163 - 174