Iterative multi-photon adaptive compensation technique for deep tissue two-photon fluorescence lifetime imaging

被引：0
|
作者：
Wang, Kexin [1 ,2 ,3 ]
Pu, Wenhui [1 ,2 ,3 ]
Qu, Junle [1 ,2 ,3 ]
Liao, Changrui [1 ,2 ,3 ]
Wang, Piping [1 ,2 ,3 ]
He, Jun [1 ,2 ,3 ]
Liu, Liwei [1 ,2 ,3 ]
机构：
[1] Shenzhen Univ, State Key Lab Radio Frequency Heterogeneous Integr, Shenzhen 518060, Peoples R China
[2] Shenzhen Univ, Coll Phys & Optoelect Engn, Key Lab Optoelect Devices & Syst Guangdong Prov, Shenzhen 518060, Peoples R China
[3] Shenzhen Univ, Coll Phys & Optoelect Engn, Minist Educ, Shenzhen 518060, Peoples R China
来源：
CHINESE OPTICS LETTERS | 2024年 / 22卷 / 04期
关键词：
adaptive optics; iterative optimization; two-photon fluorescence lifetime imaging microscopy; wavefront correction; FOCUSING LIGHT; OPTIMIZATION; MICROSCOPY; ALGORITHMS; OPTICS; SENSOR; SYSTEM;
D O I：
10.3788/COL202422.041702
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Fluorescence lifetime imaging can reveal the high-resolution structure of various biophysical and chemical parameters in a microenvironment quantitatively. However, the depth of imaging is generally limited to hundreds of micrometers due to aberration and light scattering in biological tissues. This paper introduces an iterative multi-photon adaptive compensation technique (IMPACT) into a two-photon fluorescence lifetime microscopy system to successfully overcome aberrations and multiple scattering problems in deep tissues. It shows that 400 correction modes can be achieved within 5 min, which was mainly limited by the frame rate of a spatial light modulator. This system was used for high-resolution imaging of mice brain tissue and live zebrafish, further verifying its superior performance in imaging quality and photon accumulation speed.
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页数：6
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