Iterative multi-photon adaptive compensation technique for deep tissue two-photon fluorescence lifetime imaging

被引：0
|
作者：
Wang, Kexin [1 ,2 ,3 ]
Pu, Wenhui [1 ,2 ,3 ]
Qu, Junle [1 ,2 ,3 ]
Liao, Changrui [1 ,2 ,3 ]
Wang, Piping [1 ,2 ,3 ]
He, Jun [1 ,2 ,3 ]
Liu, Liwei [1 ,2 ,3 ]
机构：
[1] Shenzhen Univ, State Key Lab Radio Frequency Heterogeneous Integr, Shenzhen 518060, Peoples R China
[2] Shenzhen Univ, Coll Phys & Optoelect Engn, Key Lab Optoelect Devices & Syst Guangdong Prov, Shenzhen 518060, Peoples R China
[3] Shenzhen Univ, Coll Phys & Optoelect Engn, Minist Educ, Shenzhen 518060, Peoples R China
来源：
CHINESE OPTICS LETTERS | 2024年 / 22卷 / 04期
关键词：
adaptive optics; iterative optimization; two-photon fluorescence lifetime imaging microscopy; wavefront correction; FOCUSING LIGHT; OPTIMIZATION; MICROSCOPY; ALGORITHMS; OPTICS; SENSOR; SYSTEM;
D O I：
10.3788/COL202422.041702
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Fluorescence lifetime imaging can reveal the high-resolution structure of various biophysical and chemical parameters in a microenvironment quantitatively. However, the depth of imaging is generally limited to hundreds of micrometers due to aberration and light scattering in biological tissues. This paper introduces an iterative multi-photon adaptive compensation technique (IMPACT) into a two-photon fluorescence lifetime microscopy system to successfully overcome aberrations and multiple scattering problems in deep tissues. It shows that 400 correction modes can be achieved within 5 min, which was mainly limited by the frame rate of a spatial light modulator. This system was used for high-resolution imaging of mice brain tissue and live zebrafish, further verifying its superior performance in imaging quality and photon accumulation speed.
引用
收藏
页数：6
相关论文
共 50 条
  • [21] Fluorescence imaging with two-photon evanescent wave excitation
    Florian Schapper
    José Tiago Gonçalves
    Martin Oheim
    European Biophysics Journal, 2003, 32 : 635 - 643
  • [22] Two-photon fluorescence probes for imaging of mitochondria and lysosomes
    Yang, Wanggui
    Chan, Pui Shan
    Chan, Miu Shan
    Li, King Fai
    Lo, Pik Kwan
    Mak, Nai Ki
    Cheah, Kok Wai
    Wong, Man Shing
    CHEMICAL COMMUNICATIONS, 2013, 49 (33) : 3428 - 3430
  • [23] Fluorescence imaging with two-photon evanescent wave excitation
    Schapper, F
    Gonçalves, JT
    Oheim, M
    EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 2003, 32 (07): : 635 - 643
  • [24] Multi-photon skin tissue imaging technology and its applications
    Ying Ya-chen
    Zhang Guang-jie
    Jia Hui-lin
    Lu Zheng-yuan
    Shi Yu-jie
    Xi Peng
    CHINESE OPTICS, 2019, 12 (01): : 104 - 111
  • [25] Two-photon deep-tissue spatially resolved mitochondrial imaging using membrane potential fluorescence fluctuations
    Tehrani, Kayvan Forouhesh
    Pendleton, Emily G.
    Southern, William M.
    Call, Jarrod A.
    Mortensen, Luke J.
    BIOMEDICAL OPTICS EXPRESS, 2018, 9 (01): : 254 - 259
  • [26] Heteroatom-Containing Organic Molecule for Two-Photon Fluorescence Lifetime Imaging and Photodynamic Therapy
    Hu, Wenbo
    Wang, Qi
    Miao, Xiaofei
    Bai, Lei
    Li, Lin
    He, Guang S.
    Li, Junzi
    Yao, Song
    He, Tingchao
    Lu, Xiaomei
    Huang, Wei
    Prasad, Paras N.
    Fan, Quli
    JOURNAL OF PHYSICAL CHEMISTRY C, 2018, 122 (36): : 20945 - 20951
  • [27] Visualizing heterogeneity of photosynthetic properties of plant leaves with two-photon fluorescence lifetime imaging microscopy
    Iermak, Ievgeniia
    Vink, Jochem
    Bader, Arjen N.
    Wientjes, Emilie
    van Amerongen, Herbert
    BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2016, 1857 (09): : 1473 - 1478
  • [28] Evaluation of the oxidative stress of psoriatic fibroblasts based on Spectral Two-photon Fluorescence Lifetime imaging
    Kapsokalyvas, Dimitrios
    Barygina, Victoria
    Cicchi, Riccardo
    Fiorillo, Claudia
    Pavone, Francesco S.
    MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XIII, 2013, 8588
  • [29] Video-rate two-photon excited fluorescence lifetime imaging system with interleaved digitization
    Dow, Ximeng Y.
    Sullivan, Shane Z.
    Muir, Ryan D.
    Simpson, Garth J.
    OPTICS LETTERS, 2015, 40 (14) : 3296 - 3299
  • [30] Deep tissue super-resolution imaging with adaptive optical two-photon multifocal structured illumination microscopy
    Zhang, Chenshuang
    Yu, Bin
    Lin, Fangrui
    Samanta, Soham
    Yu, Huanhuan
    Zhang, Wei
    Jing, Yingying
    Shang, Chunfeng
    Lin, Danying
    Si, Ke
    Gong, Wei
    Qu, Junle
    PHOTONIX, 2023, 4 (01)
12345