Iterative multi-photon adaptive compensation technique for deep tissue two-photon fluorescence lifetime imaging

被引：0

作者：

Wang, Kexin ^{[1
,2
,3
]}

Pu, Wenhui ^{[1
,2
,3
]}

Qu, Junle ^{[1
,2
,3
]}

Liao, Changrui ^{[1
,2
,3
]}

Wang, Piping ^{[1
,2
,3
]}

He, Jun ^{[1
,2
,3
]}

Liu, Liwei ^{[1
,2
,3
]}

机构：

[1] Shenzhen Univ, State Key Lab Radio Frequency Heterogeneous Integr, Shenzhen 518060, Peoples R China

[2] Shenzhen Univ, Coll Phys & Optoelect Engn, Key Lab Optoelect Devices & Syst Guangdong Prov, Shenzhen 518060, Peoples R China

[3] Shenzhen Univ, Coll Phys & Optoelect Engn, Minist Educ, Shenzhen 518060, Peoples R China

来源：

CHINESE OPTICS LETTERS | 2024年 / 22卷 / 04期

关键词：

adaptive optics; iterative optimization; two-photon fluorescence lifetime imaging microscopy; wavefront correction; FOCUSING LIGHT; OPTIMIZATION; MICROSCOPY; ALGORITHMS; OPTICS; SENSOR; SYSTEM;

D O I：

10.3788/COL202422.041702

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Fluorescence lifetime imaging can reveal the high-resolution structure of various biophysical and chemical parameters in a microenvironment quantitatively. However, the depth of imaging is generally limited to hundreds of micrometers due to aberration and light scattering in biological tissues. This paper introduces an iterative multi-photon adaptive compensation technique (IMPACT) into a two-photon fluorescence lifetime microscopy system to successfully overcome aberrations and multiple scattering problems in deep tissues. It shows that 400 correction modes can be achieved within 5 min, which was mainly limited by the frame rate of a spatial light modulator. This system was used for high-resolution imaging of mice brain tissue and live zebrafish, further verifying its superior performance in imaging quality and photon accumulation speed.

引用

页数：6

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