Regional structure-function relationships of lumbar cartilage endplates

被引:8
作者
Buchweitz, Nathan [1 ]
Sun, Yi [2 ]
Porto, Sarah Cisewski [1 ,3 ]
Kelley, Joshua [1 ]
Niu, Yipeng [4 ]
Wang, Shangping [1 ]
Meng, Zhaoxu [5 ]
Reitman, Charles [6 ]
Slate, Elizabeth [7 ]
Yao, Hai [1 ,6 ]
Wu, Yongren [1 ,6 ,8 ]
机构
[1] Clemson Univ, Dept Bioengn, Clemson, SC USA
[2] Harbin Med Univ, Affiliated Hosp 2, Dept Orthopaed, Harbin, Peoples R China
[3] Sch Hlth Sci, Coll Charleston, Charleston, SC USA
[4] NYU, Coll Art & Sci, New York, NY USA
[5] Clemson Univ, Dept Mech Engn, Clemson, SC USA
[6] Med Univ South Carolina, Dept Orthopaed & Phys Med, Charleston, SC USA
[7] Florida State Univ, Dept Stat, Tallahassee, FL USA
[8] Clemson Univ, Dept Bioengn, Clemson MUSC Bioengn Program, 68 President St BE101D MSC501, Charleston, SC 29425 USA
关键词
Low back pain; Intervertebral disc; Cartilage endplate; Biomechanics; INTERVERTEBRAL DISC; ANNULUS FIBROSUS; CONFINED COMPRESSION; ARTICULAR-CARTILAGE; NUCLEUS PULPOSUS; DEGENERATION; PERMEABILITY; CALCIFICATION; REGENERATION; DEFORMATION;
D O I
10.1016/j.jbiomech.2024.112131
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Cartilage endplates (CEPs) act as protective mechanical barriers for intervertebral discs (IVDs), yet their heterogeneous structure-function relationships are poorly understood. This study addressed this gap by characterizing and correlating the regional biphasic mechanical properties and biochemical composition of human lumbar CEPs. Samples from central, lateral, anterior, and posterior portions of the disc (n = 8/region) were mechanically tested under confined compression to quantify swelling pressure, equilibrium aggregate modulus, and hydraulic permeability. These properties were correlated with CEP porosity and glycosaminoglycan (s-GAG) content, which were obtained by biochemical assays of the same specimens. Both swelling pressure (142.79 +/- 85.89 kPa) and aggregate modulus (1864.10 +/- 1240.99 kPa) were found to be regionally dependent (p = 0.0001 and p = 0.0067, respectively) in the CEP and trended lowest in the central location. No significant regional dependence was observed for CEP permeability (1.35 +/- 0.97 * 10-16 m4/Ns). Porosity measurements correlated significantly with swelling pressure (r = -0.40, p = 0.0227), aggregate modulus (r = -0.49, p = 0.0046), and permeability (r = 0.36, p = 0.0421), and appeared to be the primary indicator of CEP biphasic mechanical properties. Second harmonic generation microscopy also revealed regional patterns of collagen fiber anchoring, with fibers inserting the CEP perpendicularly in the central region and at off-axial directions in peripheral regions. These results suggest that CEP tissue has regionally dependent mechanical properties which are likely due to the regional variation in porosity and matrix structure. This work advances our understanding of healthy baseline endplate biomechanics and lays a groundwork for further understanding the role of CEPs in IVD degeneration.
引用
收藏
页数:9
相关论文
共 50 条
[21]   Homologous structure-function relationships between native fibrocartilage and tissue engineered from MSC-seeded nanofibrous scaffolds [J].
Nerurkar, Nandan L. ;
Han, Woojin ;
Mauck, Robert L. ;
Elliott, Dawn M. .
BIOMATERIALS, 2011, 32 (02) :461-468
[22]   Structure-Function Studies of the Claudin Pore [J].
Yu, Alan S. L. .
CLAUDINS, 2010, 65 :79-95
[23]   Lamellar bone: Structure-function relations [J].
Weiner, S ;
Traub, W ;
Wagner, HD .
JOURNAL OF STRUCTURAL BIOLOGY, 1999, 126 (03) :241-255
[24]   Differential Maturation and Structure-Function Relationships in Mesenchymal Stem Cell- and Chondrocyte-Seeded Hydrogels [J].
Erickson, Isaac E. ;
Huang, Alice H. ;
Chung, Cindy ;
Li, Ryan T. ;
Burdick, Jason A. ;
Mauck, Robert L. .
TISSUE ENGINEERING PART A, 2009, 15 (05) :1041-1052
[25]   Structure-Function Correlation of the Human Central Retina [J].
Issa, Peter Charbel ;
Troeger, Eric ;
Finger, Robert ;
Holz, Frank G. ;
Wilke, Robert ;
Scholl, Hendrik P. N. .
PLOS ONE, 2010, 5 (09) :1-9
[26]   Degeneration alters structure-function relationships at multiple length-scales and across interfaces in human intervertebral discs [J].
Ashinsky, Beth G. ;
Gullbrand, Sarah E. ;
Wang, Chao ;
Bonnevie, Edward D. ;
Han, Lin ;
Mauck, Robert L. ;
Smith, Harvey E. .
JOURNAL OF ANATOMY, 2021, 238 (04) :986-998
[27]   The Yucatan Minipig Temporomandibular Joint Disc Structure-Function Relationships Support Its Suitability for Human Comparative Studies [J].
Vapniarsky, Natalia ;
Aryaei, Ashkan ;
Arzi, Boaz ;
Hatcher, David C. ;
Hu, Jerry C. ;
Athanasiou, Kyriacos A. .
TISSUE ENGINEERING PART C-METHODS, 2017, 23 (11) :700-709
[28]   NMJ-morph reveals principal components of synaptic morphology influencing structure-function relationships at the neuromuscular junction [J].
Jones, Ross A. ;
Reich, Caitlan D. ;
Dissanayake, Kosala N. ;
Kristmundsdottir, Fanney ;
Findlater, Gordon S. ;
Ribchester, Richard R. ;
Simmen, Martin W. ;
Gillingwater, Thomas H. .
OPEN BIOLOGY, 2016, 6 (12)
[29]   Composition and dynamics of articular cartilage: Structure, function, and maintaining healthy state [J].
Cohen, NP ;
Foster, RJ ;
Mow, VC .
JOURNAL OF ORTHOPAEDIC & SPORTS PHYSICAL THERAPY, 1998, 28 (04) :203-215
[30]   Structure and function of the lumbar intervertebral disk in health, aging, and pathologic conditions [J].
Lundon, K ;
Bolton, K .
JOURNAL OF ORTHOPAEDIC & SPORTS PHYSICAL THERAPY, 2001, 31 (06) :291-303