Protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging

被引：0
|
作者：
Park, Seungbin [1 ]
Lipton, Megan [1 ]
Sun, Yujiao J. [2 ]
Dadarlat, Maria [1 ]
机构：
[1] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA
[2] UCL, Inst Ophthalmol, 11-43 Bath St, London EC1V 9EL, England
来源：
STAR PROTOCOLS | 2024年 / 5卷 / 02期
关键词：
PYRAMIDAL TRACT CELLS; MICROSTIMULATION; EXCITATION; ACTIVATION; SENSATIONS; NEURONS; DRIVER; LINES;
D O I：
10.1016/j.xpro.2024.103027
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Electrical stimulation provides a clinically viable approach for treating neurological disorders. Here, we present a protocol for recording neural activity evoked by electrical stimulation in mice using two -photon calcium imaging. We detail steps for chronically implanting a head fixation bar, a stimulating electrode, and a glass imaging window. We additionally describe the procedures for viral injections and awake head -fixed recordings. For complete details on the use and execution of this protocol, please refer to Dadarlat et al. 1
引用
收藏
页数：20
相关论文
共 50 条
  • [1] Improved deep two-photon calcium imaging in vivo
    Birkner, Antje
    Tischbirek, Carsten H.
    Konnerth, Arthur
    CELL CALCIUM, 2017, 64 : 29 - 35
  • [2] Two-photon calcium imaging of neuronal and astrocytic responses: the influence of electrical stimulus parameters and calcium signaling mechanisms
    Ma, Zengguang
    Wei, Liangpeng
    Du, Xiaolang
    Hou, Shaowei
    Chen, Feng
    Jiao, Qingyan
    Liu, Aili
    Liu, Shujing
    Wang, Junsong
    Shen, Hui
    JOURNAL OF NEURAL ENGINEERING, 2021, 18 (04)
  • [3] Two-photon calcium imaging of seizures in awake, head-fixed mice
    Somarowthu, Ala
    Goff, Kevin M.
    Goldberg, Ethan M.
    CELL CALCIUM, 2021, 96
  • [4] Two-photon microscopy for intracutaneous imaging of stem cell activity in mice
    Huang, Sixia
    Rompolas, Panteleimon
    EXPERIMENTAL DERMATOLOGY, 2017, 26 (05) : 379 - 383
  • [5] Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo
    Wu, Jianglai
    Liang, Yajie
    Chen, Shuo
    Hsu, Ching-Lung
    Chavarha, Mariya
    Evans, Stephen W.
    Shi, Dongqing
    Lin, Michael Z.
    Tsia, Kevin K.
    Ji, Na
    NATURE METHODS, 2020, 17 (03) : 287 - +
  • [6] Protocol for in vivo recording of neural activity in deep structures of mice brain via gradient lenses by calcium imaging
    Cherepanov, Stanislav
    Mollard, Patrice
    Martin, Agnes O.
    STAR PROTOCOLS, 2025, 6 (01):
  • [7] Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae
    Muto, Akira
    Kawakami, Koichi
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2018, (136):
  • [8] Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds
    Clough, Mitchell
    Chen, Ichun Anderson
    Park, Seong-Wook
    Ahrens, Allison M.
    Stirman, Jeffrey N.
    Smith, Spencer L.
    Chen, Jerry L.
    NATURE COMMUNICATIONS, 2021, 12 (01)
  • [9] Kilohertz two-photon brain imaging in awake mice
    Zhang, Tong
    Hernandez, Oscar
    Chrapkiewicz, Radoslaw
    Shai, Adam
    Wagner, Mark J.
    Zhang, Yanping
    Wu, Cheng-Hsun
    Li, Jin Zhong
    Inoue, Masatoshi
    Gong, Yiyang
    Ahanonu, Biafra
    Zeng, Hongkui
    Bito, Haruhiko
    Schnitzer, Mark J.
    NATURE METHODS, 2019, 16 (11) : 1119 - +
  • [10] Optogenetic Imaging of Protein Activity Using Two-Photon Fluorescence Lifetime Imaging Microscopy
    Murakoshi, Hideji
    OPTOGENETICS: LIGHT-SENSING PROTEINS AND THEIR APPLICATIONS IN NEUROSCIENCE AND BEYOND, 2ND EDITION, 2021, 1293 : 295 - 308
12345