Intravital microscopy of satellite cell dynamics and their interaction with myeloid cells during skeletal muscle regeneration

被引:4
作者
He, Yingzhu [1 ]
Heng, Youshan [2 ]
Qin, Zhongya [1 ]
Wei, Xiuqing [2 ]
Wu, Zhenguo [2 ]
Qu, Jianan [1 ,3 ]
机构
[1] Hong Kong Univ Sci & Technol, Dept Elect & Comp Engn, Clear Water Bay,Kowloon, Hong Kong, Peoples R China
[2] Hong Kong Univ Sci & Technol, Div Life Sci, Clear Water Bay,Kowloon, Hong Kong, Peoples R China
[3] Hong Kong Univ Sci & Technol, State Key Lab Mol Neurosci, Clear Water Bay,Kowloon, Hong Kong, Peoples R China
来源
SCIENCE ADVANCES | 2023年 / 9卷 / 42期
关键词
2ND-HARMONIC GENERATION; STEM-CELLS; IMAGING REVEALS; T-CELLS; MACROPHAGES; ACTIVATION; TRANSITION; QUIESCENCE; MONOCYTES; APOPTOSIS;
D O I
10.1126/sciadv.adi1891
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Skeletal muscle regeneration requires the highly coordinated cooperation of muscle satellite cells (MuSCs) with other cellular components. Upon injury, myeloid cells populate the wound site, concomitant with MuSC activation. However, detailed analysis of MuSC-myeloid cell interaction is hindered by the lack of suitable live animal imaging technology. Here, we developed a dual-laser multimodal nonlinear optical microscope platform to study the dynamics of MuSCs and their interaction with nonmyogenic cells during muscle regeneration. Using three-dimensional time-lapse imaging on live reporter mice and taking advantages of the autofluorescence of reduced nicotinamide adenine dinucleotide (NADH), we studied the spatiotemporal interaction between nonmyogenic cells and muscle stem/progenitor cells during MuSC activation and proliferation. We discovered that their cell-cell contact was transient in nature. Moreover, MuSCs could activate with notably reduced infiltration of neutrophils and macrophages, and their proliferation, although dependent on macrophages, did not require constant contact with them. These findings provide a fresh perspective on myeloid cells' role during muscle regeneration.
引用
收藏
页数:18
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