Functional Characterization of the Paeonia ostii P5CS Gene under Drought Stress

被引:1
|
作者
Luan, Yuting [1 ]
An, Honglei [1 ]
Chen, Zijie [1 ]
Zhao, Daqiu [1 ]
Tao, Jun [1 ,2 ]
机构
[1] Yangzhou Univ, Coll Hort & Landscape Architecture, Yangzhou 225009, Peoples R China
[2] Yangzhou Univ, Joint Int Res Lab Agr & Agriprod Safety, Minist Educ China, Yangzhou 225009, Peoples R China
来源
PLANTS-BASEL | 2024年 / 13卷 / 15期
关键词
Paeonia ostii; P5CS; proline; drought stress; DELTA(1)-PYRROLINE-5-CARBOXYLATE SYNTHETASE P5CS; PROLINE ACCUMULATION; PAEONIA-OSTII; SALT-STRESS; GLYCINE BETAINE; COLD TOLERANCE; EXPRESSION; TOBACCO; ROLES; TRANSFORMATION;
D O I
10.3390/plants13152145
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
With persistent elevation in global temperature, water scarcity becomes a major threat to plant growth and development, yield security, agricultural sustainability, and food production. Proline, as a key osmolyte and antioxidant, plays a critical role in regulating drought tolerance in plants, especially its key biosynthetic enzyme, delta-1-pyrroline-5-carboxylate synthase (P5CS), which always positively responds to drought stress. As an important woody oil crop, the expansion of Paeonia ostii cultivation needs to address the issue of plant drought tolerance. Here, we isolated a PoP5CS gene from P. ostii, with an open reading frame of 1842 bp encoding 613 amino acids. PoP5CS expression progressively increased in response to increasing drought stress, and it was localized in the cytoplasm. Silencing of PoP5CS in P. ostii reduced drought tolerance, accompanied by decreased proline content, elevated reactive oxygen species (ROS) accumulation, and increased relative electrical conductivity (REC) and malondialdehyde (MDA) levels. Conversely, overexpression of PoP5CS in Nicotiana tabacum plants enhanced drought resistance, manifested by increased proline levels, reduced ROS accumulation, and lower REC and MDA contents. This study isolates PoP5CS from P. ostii and validates its role in regulating drought tolerance, providing valuable genetic resources and theoretical insights for the development of drought-resistant P. ostii cultivars.
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页数:14
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