Evaluation of different molecular systems for detection and quantification of SARS-CoV-2 RNA from wastewater samples

被引：1

作者：

Morecchiato, Fabio ^{[1
]}

Coppi, Marco ^{[1
,2
]}

Niccolai, Claudia ^{[1
,2
]}

Antonelli, Alberto ^{[1
,2
]}

Di Gloria, Leandro ^{[3
]}

Cala, Piergiuseppe ^{[4
]}

Mancuso, Fabrizio ^{[5
]}

Ramazzotti, Matteo ^{[3
]}

Lotti, Tommaso ^{[6
]}

Lubello, Claudio ^{[6
]}

Rossolini, Gian Maria ^{[1
,2
]}

机构：

[1] Univ Florence, Dept Expt & Clin Med DMSC, Largo Brambilla 3, I-50134 Florence, FI, Italy

[2] Careggi Univ Hosp, Microbiol & Virol Unit, Largo Brambilla 3, I-50134 Florence, FI, Italy

[3] Univ Florence, Dept Expt Biomed & Clin Sci Mario Serio SBSC, Viale Morgagni 50, I-50134 Florence, FI, Italy

[4] Local Hlth Author Tuscany Ctr, Tuscany Reg, Dept Prevent, Via S Salvi 12, I-50135 Florence, FI, Italy

[5] Ingegnerie Toscane, Area R&D, Via Bellatalla 1, I-56121 Pisa, PI, Italy

[6] Univ Florence, Dept Civil & Environm Engn DICEA, Via S Marta 3, I-50139 Florence, FI, Italy

来源：

JOURNAL OF VIROLOGICAL METHODS | 2024年 / 328卷

关键词：

SARS-CoV-2; Wastewater; Wastewater -based epidemiology; Environmental surveillance; Reverse transcription real-time PCR; Droplet digital PCR; Digital PCR; SURVEILLANCE;

D O I：

10.1016/j.jviromet.2024.114956

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Wastewater-based epidemiology has proved to be a suitable approach for tracking the spread of epidemic agents including SARS-CoV-2 RNA. Different protocols have been developed for quantitative detection of SARS-CoV-2 RNA from wastewater samples, but little is known on their performance. In this study we compared three protocols based on Reverse Transcription Real Time-PCR (RT-PCR) and one based on Droplet Digital PCR (ddPCR) for SARS-CoV-2 RNA detection from 35 wastewater samples. Overall, SARS-CoV-2 RNA was detected by at least one method in 85.7 % of samples, while 51.4 %, 22.8 % and 8.6 % resulted positive with two, three or all four methods, respectively. Protocols based on commercial RT-PCR assays and on Droplet Digital PCR showed an overall higher sensitivity vs. an in-house assay. The use of more than one system, targeting different genes, could be helpful to increase detection sensitivity.

引用

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