Epigenetic markers of tooth eruption - DNA methylation and histone acetylation

被引：0

作者：

Westerlund, Anna ^{[1
]}

Shikhan, Asal ^{[2
]}

Sabel, Nina ^{[3
]}

Asa'ad, Farah ^{[4
]}

Larsson, Lena ^{[4
]}

机构：

[1] Univ Gothenburg, Inst Odontol, Sahlgrenska Acad, Dept Orthodont, Gothenburg, Sweden

[2] Univ Gothenburg, Inst Odontol, Sahlgrenska Acad, Dept Periodontol, Gothenburg, Sweden

[3] Univ Gothenburg, Inst Odontol, Sahlgrenska Acad, Dept Pediat Dent, Gothenburg, Sweden

[4] Univ Gothenburg, Inst Odontol, Sahlgrenska Acad, Dept Oral Biochem, Gothenburg, Sweden

来源：

EUROPEAN JOURNAL OF ORAL SCIENCES | 2024年 / 132卷 / 04期

关键词：

dental follicle; DNA methylation; ectopic canines; epigenetic; histone acetylation; tooth impaction; DENTAL FOLLICLE; OSTEOGENIC DIFFERENTIATION; DEACETYLASE INHIBITORS; GENE-EXPRESSION; MAMMALIAN DNA; CELLS; BONE; OSTEOCLASTOGENESIS; 5-HYDROXYMETHYLCYTOSINE; PHOSPHATASE;

D O I：

10.1111/eos.13005

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

The present study aimed to evaluate whether epigenetic markers are expressed in the dental follicles surrounding ectopically erupting teeth. Twenty-one dental follicles were collected in 20 adolescent children through surgical exposure of ectopic teeth. The epigenetic modifications of DNA methylation and histone acetylation were evaluated by immunohistochemistry. The results showed cells positive for DNA-methyltransferase 1 (DNMT1), DNA methyltransferase 3 beta (DNMT3B), ten-eleven translocation-2 (TET2), acetyl-histone H3 (AcH3), acetyl-histone H4 (AcH4), 5-methylcytosine (5mC), and 5-hydroxymethylcytosine (5hmC) were present in all the samples. The levels of epigenetic markers representing active chromatin (5hmC, AcH3, AcH4, and TET2) were statistically significantly higher than those of markers representing inactive chromatin (5mC, DNMT3B, DNMT1). In conclusion, follicles in ectopic teeth display major epigenetic modifications. In the follicles, epigenetic markers associated with the activation of bone-related genes are more abundant than markers associated with the inactivation of bone-related genes.

引用

页数：11

共 50 条

[31] Epigenetic diagnostics of cancer — the application of DNA methylation markers
Jaroslaw Paluszczak
Wanda Baer-Dubowska
Journal of Applied Genetics, 2006, 47 : 365 - 375
[32] Epigenetic diagnostics of cancer - the application of DNA methylation markers
Paluszczak, Jaroslaw
Baer-Dubowska, Wanda
JOURNAL OF APPLIED GENETICS, 2006, 47 (04) : 365 - 375
[33] Epigenetic Modifications in Distinction: Histone versus DNA Methylation in ESCs
Huang, Kevin
Fan, Guoping
CELL STEM CELL, 2011, 8 (06) : 604 - 605
[34] A Structural Perspective on Readout of Epigenetic Histone and DNA Methylation Marks
Patel, Dinshaw J.
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY, 2016, 8 (03):
[35] Glucocorticoid-mediated transrepression is regulated by histone acetylation and DNA methylation
Kagoshima, M
Wilcke, T
Ito, K
Tsaprouni, L
Barnes, PJ
Punchard, N
Adcock, IM
EUROPEAN JOURNAL OF PHARMACOLOGY, 2001, 429 (1-3) : 327 - 334
[36] Cell-specific epigenetic regulation of ChM-I gene expression: Crosstalk between DNA methylation and histone acetylation
Aoyama, Tomoki
Okamoto, Takeshi
Kohno, Yoshiki
Fukiage, Kenichi
Otsuka, Seiji
Furu, Moritoshi
Ito, Kinya
Jin, Yonghui
Nagayama, Satoshi
Nakayama, Tomitaka
Nakamura, Takashi
Toguchida, Junya
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2008, 365 (01) : 124 - 130
[37] Epigenetic control by histone methylation
Jenuwein, T.
FEBS JOURNAL, 2011, 278 : 5 - 5
[38] Epigenetic regulation by histone methylation and histone variants
Cheung, P
Lau, P
MOLECULAR ENDOCRINOLOGY, 2005, 19 (03) : 563 - 573
[39] Role of Histone Acetylation and Methylation in Obesity
Xu L.
Yeung M.H.Y.
Yau M.Y.C.
Lui P.P.Y.
Wong C.-M.
Current Pharmacology Reports, 2019, 5 (3) : 196 - 203
[40] Epigenetic interplay between histone modifications and DNA methylation in gene silencing
Vaissiere, Thomas
Sawan, Carla
Herceg, Zdenko
MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH, 2008, 659 (1-2) : 40 - 48

← 1 2 3 4 5 →