Epigenetic markers of tooth eruption - DNA methylation and histone acetylation

被引：0

作者：

Westerlund, Anna ^{[1
]}

Shikhan, Asal ^{[2
]}

Sabel, Nina ^{[3
]}

Asa'ad, Farah ^{[4
]}

Larsson, Lena ^{[4
]}

机构：

[1] Univ Gothenburg, Inst Odontol, Sahlgrenska Acad, Dept Orthodont, Gothenburg, Sweden

[2] Univ Gothenburg, Inst Odontol, Sahlgrenska Acad, Dept Periodontol, Gothenburg, Sweden

[3] Univ Gothenburg, Inst Odontol, Sahlgrenska Acad, Dept Pediat Dent, Gothenburg, Sweden

[4] Univ Gothenburg, Inst Odontol, Sahlgrenska Acad, Dept Oral Biochem, Gothenburg, Sweden

来源：

EUROPEAN JOURNAL OF ORAL SCIENCES | 2024年 / 132卷 / 04期

关键词：

dental follicle; DNA methylation; ectopic canines; epigenetic; histone acetylation; tooth impaction; DENTAL FOLLICLE; OSTEOGENIC DIFFERENTIATION; DEACETYLASE INHIBITORS; GENE-EXPRESSION; MAMMALIAN DNA; CELLS; BONE; OSTEOCLASTOGENESIS; 5-HYDROXYMETHYLCYTOSINE; PHOSPHATASE;

D O I：

10.1111/eos.13005

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

The present study aimed to evaluate whether epigenetic markers are expressed in the dental follicles surrounding ectopically erupting teeth. Twenty-one dental follicles were collected in 20 adolescent children through surgical exposure of ectopic teeth. The epigenetic modifications of DNA methylation and histone acetylation were evaluated by immunohistochemistry. The results showed cells positive for DNA-methyltransferase 1 (DNMT1), DNA methyltransferase 3 beta (DNMT3B), ten-eleven translocation-2 (TET2), acetyl-histone H3 (AcH3), acetyl-histone H4 (AcH4), 5-methylcytosine (5mC), and 5-hydroxymethylcytosine (5hmC) were present in all the samples. The levels of epigenetic markers representing active chromatin (5hmC, AcH3, AcH4, and TET2) were statistically significantly higher than those of markers representing inactive chromatin (5mC, DNMT3B, DNMT1). In conclusion, follicles in ectopic teeth display major epigenetic modifications. In the follicles, epigenetic markers associated with the activation of bone-related genes are more abundant than markers associated with the inactivation of bone-related genes.

引用

页数：11

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