Huangqin Qingre Chubi Capsule improves rheumatoid arthritis accompanied depression through the Wnt1/ß-catenin signaling pathway

被引：3

作者：

Li, Chen ^{[1
]}

Xue, Qiuyun ^{[1
]}

Li, Hui ^{[1
]}

Peng, Yanhui ^{[1
]}

Wu, Yajie ^{[1
]}

Yuan, Meiling ^{[1
]}

Duan, Qiangjun ^{[2
]}

Hong, Xinghui ^{[2
]}

Chen, Guangliang ^{[1
]}

Liao, Faxue ^{[1
,3
,4
]}

Wu, Peng ^{[1
,5
]}

Miao, Chenggui ^{[1
,6
,7
]}

机构：

[1] Anhui Univ Chinese Med, Sch Integrated Chinese & Western Med, Dept Pharmacol, 1 Qianjiang Rd, Hefei 230012, Anhui, Peoples R China

[2] Anhui Univ Chinese Med, Dept Expt Teaching Ctr, Sch Integrated Chinese & Western Med, Hefei 230012, Peoples R China

[3] Anhui Med Univ, Affiliated Hosp 1, Hefei, Peoples R China

[4] Anhui Publ Hlth Clin Ctr, Hefei, Peoples R China

[5] Anhui Univ Chinese Med, Sch Integrated Chinese & Western Med, Dept Anat, Hefei 230012, Peoples R China

[6] Anhui Univ Chinese Med, Inst Prevent & Treatment Rheumatoid Arthrit, Hefei, Anhui, Peoples R China

[7] Univ Hong Kong, Li Ka Shing Fac Med, Sch Chinese Med, Hong Kong, Peoples R China

来源：

INTERNATIONAL IMMUNOPHARMACOLOGY | 2024年 / 138卷

基金：

中国国家自然科学基金;

关键词：

Rheumatoid arthritis; RA accompanied depression; Huangqin Qingre Chubi Capsule; Wnt1/ss-catenin signal pathway; Network pharmacology; STRESS-INDUCED DEPRESSION; NETWORK PHARMACOLOGY; BEHAVIOR; RATS;

D O I：

10.1016/j.intimp.2024.112474

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Aim of the study: Research on the mechanism of Huangqin Qingre Chubi Capsules (HQC) in improving rheumatoid arthritis accompanied depression (RA-dep) model rats. Methods: We employed real -time qPCR (RT-qPCR), western blotting (WB), confocal microscopy, bioinformatics, and other methods to investigate the anti-RA-dep effects of HQC and its underlying mechanisms. Results: HQC alleviated the pathological indexes of inflammation and depression in RA-dep model rats, decreased the levels of tumor necrosis factor (TNF)-a, interleukin (IL) -1 ss and IL -6, increased the levels of norepinephrine (NE) and serotonin (5-HT), and improved the injury of hippocampus. The analysis of network pharmacology suggests that HQC may target the Wnt/ ss- catenin pathway in the treatment of RA-dep. Furthermore, molecular dynamics simulations revealed a strong affinity between HQC and the Wnt1 molecule. RT-qPCR and Western Blot (WB) experiments confirmed the critical role of the Wnt1/ ss- catenin signaling pathway in the treatment of RAdep model rats with HQC. In vitro, the HQC drug-containing serum (HQC-serum) activates the Wnt1/ ss- cat- enin signaling pathway in hippocampal cells and, in conjunction with Wnt1, ameliorates RA-dep. In summary, HQC exerts its anti-inflammatory and antidepressant effects in the treatment of RA-dep by binding to Wnt1 and regulating the Wnt1/ ss- catenin signaling pathway. Conclusions: HQC improved the inflammatory reaction and depression-like behavior of RA-dep model rats by activating Wnt1/ ss- catenin signal pathway. This study revealed a new pathogenesis of RA-dep and contributes to the clinical promotion of HQC in the treatment of RA-dep.

引用

页数：12