Advances in Quantification and Site Stoichiometry Analysis Methods for Phosphorylated Proteins

The post -translational modification of proteins is a key mechanism that imparts physiological functions to proteins, among which reversible phosphorylation modifications play a pivotal role in many biological processes. Aberrant changes in phosphorylation are often closely associated with various major disease processes. In recent years, with the aid of proteomic technologies and methods, high -throughput, high -precision qualitative and quantitative approaches for phosphorylated proteins have rapidly advanced. This article reviews the research progress of phosphorylated protein quantification and chemical proteomics analysis methods based on the "bottomup" strategy, including phosphopeptide enrichment methods, mass spectrometry fragmentation methods, quantification analysis methods and phosphorylation site stoichiometry, and discusses the development trend of quantification and stoichiometric analysis methods for phosphorylated proteins.