Production of grains with ultra-low heavy metal accumulation by pyramiding novel Alleles of OsNramp5 and OsLsi2 in two-line hybrid rice

被引：4

作者：

Hu, Shikai ^{[1
]}

Zhou, Liang ^{[1
]}

Wang, Jingxin ^{[1
]}

Mawia, Amos Musyoki ^{[1
]}

Hui, Suozhen ^{[1
]}

Xu, Bo ^{[1
]}

Jiao, Guiai ^{[1
]}

Sheng, Zhonghua ^{[1
]}

Shao, Gaoneng ^{[1
]}

Wei, Xiangjin ^{[1
]}

Wang, Ling ^{[1
]}

Xie, Lihong ^{[1
]}

Zhao, Fengli ^{[1
]}

Tang, Shaoqing ^{[1
]}

Hu, Peisong ^{[1
]}

机构：

[1] China Natl Rice Res Inst, State Key Lab Rice Biol & Breeding, Hangzhou, Peoples R China

来源：

PLANT BIOTECHNOLOGY JOURNAL | 2024年 / 22卷 / 10期

基金：

中国国家自然科学基金;

关键词：

Ultra-low heavy metal accumulation; grain safety; two-line hybrid rice; gene pyramiding; cadmium; arsenic; TRANSPORTER; MANGANESE;

D O I：

10.1111/pbi.14414

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Ensuring rice yield and grain safety quality are vital for human health. In this study, we developed two-line hybrid rice (TLHR) with ultra-low grain cadmium (Cd) and arsenic (As) accumulation by pyramiding novel alleles of OsNramp5 and OsLsi2. We first generated low Cd accumulation restorer (R) lines by editing OsNramp5, OsLCD, and OsLCT in japonica and indica. After confirming that OsNramp5 was most efficient in reducing Cd, we edited this gene in C815S, a genic male sterile line (GMSL), and screened it for alleles with low Cd accumulation. Next, we generated R and GMSL lines with low As accumulation by editing OsLsi2 in a series of YK17 and C815S lines. When cultivated in soils that were heavily polluted with Cd and As, the edited R, GMSL, and TLHR plants showed significantly reduced heavy metal accumulation, while maintaining a relatively stable yield potential. This study provides an effective scheme for the safe production of grains in As- and/or Cd-polluted paddy fields.

引用

页码：2921 / 2931

页数：11