The Genetic Basis of Composite Spike Form in Barley and 'Miracle-Wheat'

被引：117

作者：

Poursarebani, Naser ^{[1
]}

Seidensticker, Tina ^{[1
]}

Koppolu, Ravi ^{[1
]}

Trautewig, Corinna ^{[1
]}

Gawronski, Piotr ^{[1
]}

Bini, Federica ^{[1
]}

Govind, Geetha ^{[1
]}

Rutten, Twan ^{[1
]}

Sakuma, Shun ^{[2
]}

Tagiri, Akemi ^{[2
]}

Wolde, Gizaw M. ^{[1
]}

Youssef, Helmy M. ^{[1
,3
]}

Battal, Abdulhamit ^{[4
,5
]}

Ciannamea, Stefano ^{[6
]}

Fusca, Tiziana ^{[6
]}

Nussbaumer, Thomas ^{[7
]}

Pozzi, Carlo ^{[6
]}

Boerner, Andreas ^{[1
]}

Lundqvist, Udda ^{[8
]}

Komatsuda, Takao ^{[2
]}

Salvi, Silvio ^{[9
]}

Tuberosa, Roberto ^{[9
]}

Uauy, Cristobal ^{[5
]}

Sreenivasulu, Nese ^{[1
]}

Rossini, Laura ^{[6
,10
]}

Schnurbusch, Thorsten ^{[1
]}

机构：

[1] Leibniz Inst Plant Genet & Crop Plant Res, D-06466 Ot Gatersleben, Stadt Seeland, Germany

[2] Natl Inst Agrobiol Sci, Plant Genome Res Unit, Tsukuba, Ibaraki 3058602, Japan

[3] Cairo Univ, Fac Agr, Giza 12613, Egypt

[4] John Innes Ctr, Dept Crop Genet, Norwich NR4 7UH, Norfolk, England

[5] Middle E Tech Univ, Biol Sci, TR-06800 Ankara, Turkey

[6] Parco Tecnol Padano, I-26900 Lodi, Italy

[7] Helmholtz Ctr Munich, Plant Genome & Syst Biol, D-85764 Neuherberg, Germany

[8] Nord Genet Resource Ctr, SE-23053 Alnarp, Sweden

[9] Univ Bologna, Dipartimento Sci Agr, I-40127 Bologna, Italy

[10] Univ Milan, DiSAA, I-20133 Milan, Italy

来源：

GENETICS | 2015年 / 201卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

'Miracle-Wheat'; 'Wunder-Weizen'; 'Compositum-Barley'; inflorescence branching; yield potential; SUPERNUMERARY SPIKELETS; MERISTEM IDENTITY; FLORAL MERISTEM; FRIZZY-PANICLE; ZINC-FINGER; RICE; DOMESTICATION; ARABIDOPSIS; INFLORESCENCE; PROTEIN;

D O I：

10.1534/genetics.115.176628

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Inflorescences of the tribe Triticeae, which includes wheat (Triticum sp. L.) and barley (Hordeum vulgare L.) are characterized by sessile spikelets directly borne on the main axis, thus forming a branchless spike. 'Compositum-Barley' and tetraploid 'Miracle-Wheat' (T. turgidum convar. compositum (L.f.) Filat.) display noncanonical spike-branching in which spikelets are replaced by lateral branch-like structures resembling small-sized secondary spikes. As a result of this branch formation 'Miracle-Wheat' produces significantly more grains per spike, leading to higher spike yield. In this study, we first isolated the gene underlying spike-branching in 'Compositum-Barley,' i.e., compositum 2 (com2). Moreover, we found that COM2 is orthologous to the branched head(t) (bh(t)) locus regulating spike branching in tetraploid 'Miracle-Wheat.' Both genes possess orthologs with similar functions in maize BRANCHED SILKLESS 1 (BD1) and rice FRIZZY PANICLE/BRANCHED FLORETLESS 1 (FZP/BFL1) encoding AP2/ERF transcription factors. Sequence analysis of the bht locus in a collection of mutant and wild-type tetraploid wheat accessions revealed that a single amino acid substitution in the DNA-binding domain gave rise to the domestication of 'Miracle-Wheat.' mRNA in situ hybridization, microarray experiments, and independent qRT-PCR validation analyses revealed that the branch repression pathway in barley is governed through the spike architecture gene Six-rowed spike 4 regulating COM2 expression, while HvIDS1 (barley ortholog of maize INDETERMINATE SPIKELET 1) is a putative downstream target of COM2. These findings presented here provide new insights into the genetic basis of spike architecture in Triticeae, and have disclosed new targets for genetic manipulations aiming at boosting wheat's yield potential.

引用

页码：155 / +

页数：39

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