Inside of the burst-phase intermediate of a protein folding. Hydration of hydrophobic groups

被引：0
作者：
Bolonova, Elena I. [1 ,2 ]
Melnik, Tatiana N. [1 ]
Potekhin, Sergey A. [1 ]
机构：
[1] Russian Acad Sci, Inst Prot Res, Pushchino 142290, Moscow Reg, Russia
[2] Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Hung Hom, Kowloon, Hong Kong, Peoples R China
来源：
BIOPHYSICAL CHEMISTRY | 2024年 / 312卷
关键词：
Protein folding; Burst-phase intermediate; Dehydration of aliphatic groups; Isothermal titration calorimetry; Molten globule state & sdot; ISOTHERMAL TITRATION CALORIMETRY; MOLTEN GLOBULE INTERMEDIATE; POLY-N-ISOPROPYLACRYLAMIDE; TRANSITIONS; LYSOZYME; PATHWAY; STATE; EQUILIBRIUM; KINETICS; COLLAPSE;
D O I：
10.1016/j.bpc.2024.107268
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The thermal effect of the formation of the "burst-phase" folding intermediate has been studied using a titration calorimeter. It is shown that, unlike the total thermal effect of native structure formation, it can be both positive and negative depending on the temperature. The reasons for this paradoxical behavior are analyzed. A conclusion is drawn about the leading role of dehydration of non-polar groups in the first stage of folding.
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页数：6
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共 46 条
  • [1] Evidence for Dry Molten Globule-Like Domains in the pH-Induced Equilibrium Folding Intermediate of a Multidomain Protein
    Acharya, Nirbhik
    Mishra, Prajna
    Jha, Santosh Kumar
    [J]. JOURNAL OF PHYSICAL CHEMISTRY LETTERS, 2016, 7 (01): : 173 - 179
  • [2] On-pathway versus off-pathway folding intermediates
    Baldwin, RL
    [J]. FOLDING & DESIGN, 1996, 1 (01): : R1 - R8
  • [3] PULSED H/D-EXCHANGE STUDIES OF FOLDING INTERMEDIATES
    BALDWIN, RL
    [J]. CURRENT OPINION IN STRUCTURAL BIOLOGY, 1993, 3 (01) : 84 - 91
  • [4] Calorimetry of enzyme-catalyzed reactions
    Bianconi, M. Lucia
    [J]. BIOPHYSICAL CHEMISTRY, 2007, 126 (1-3) : 59 - 64
  • [5] Bruscolini P, 2002, MACROMOL SYMP, V181, P261, DOI 10.1002/1521-3900(200205)181:1<261::AID-MASY261>3.0.CO
  • [6] 2-Z
  • [7] kinITC: A New Method for Obtaining Joint Thermodynamic and Kinetic Data by Isothermal Titration Calorimetry
    Burnouf, Dominique
    Ennifar, Eric
    Guedich, Sondes
    Puffer, Barbara
    Hoffmann, Guillaume
    Bec, Guillaume
    Disdier, Francois
    Baltzinger, Mireille
    Dumas, Philippe
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2012, 134 (01) : 559 - 565
  • [8] Order-disorder conformational transitions of N-isopropylacrylamide-sodium styrene sulfonate copolymers in aqueous solutions
    Burova, Tatiana V.
    Grinberg, Natalia V.
    Grinberg, Valerij Ya.
    Ang, Yecang T.
    Zhang, Guangzhao
    Khokhlov, Alexei R.
    [J]. MACROMOLECULES, 2008, 41 (16) : 5981 - 5984
  • [9] KINETIC RESOLUTION OF PEPTIDE-BOND AND SIDE-CHAIN FAR-UV CIRCULAR-DICHROISM DURING THE FOLDING OF HEN EGG-WHITE LYSOZYME
    CHAFFOTTE, AF
    GUILLOU, Y
    GOLDBERG, ME
    [J]. BIOCHEMISTRY, 1992, 31 (40) : 9694 - 9702
  • [10] Structure of the acid state of Escherichia coli ribonuclease HI
    Dabora, JM
    Pelton, JG
    Marqusee, S
    [J]. BIOCHEMISTRY, 1996, 35 (37) : 11951 - 11958
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