UV-B Radiation Disrupts Membrane Lipid Organization and Suppresses Protein Mobility of GmNARK in Arabidopsis

被引:0
|
作者
Liu, Qiulin [1 ,2 ]
Wang, Tianyu [1 ,2 ]
Ke, Meiyu [1 ,2 ]
Qian, Chongzhen [3 ]
Li, Jiejie [4 ]
Huang, Xi [3 ]
Gao, Zhen [1 ,2 ]
Chen, Xu [2 ]
Tu, Tianli [2 ]
机构
[1] Fujian Agr & Forestry Univ, Coll Life Sci, Fujian Prov Key Lab Haixia Appl Plant Syst Biol, Fuzhou 350002, Peoples R China
[2] Fujian Agr & Forestry Univ, Haixia Inst Sci & Technol, Hort Plant Biol & Metabol Ctr, Fuzhou 350002, Peoples R China
[3] Xiamen Univ, Sch Life Sci, State Key Lab Cellular Stress Biol, Xiamen 361102, Peoples R China
[4] Beijing Normal Univ, Coll Life Sci, Beijing Key Lab Gene Resource & Mol Dev, Beijing 100875, Peoples R China
来源
PLANTS-BASEL | 2024年 / 13卷 / 11期
关键词
UV-B; GmNARK; endocytosis; lipid organization; STRESS; PLANTS; RAFTS; ACCLIMATION; RESPONSES; CHOLESTEROL; ENDOCYTOSIS; MODEL;
D O I
10.3390/plants13111536
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
While it is well known that plants interpret UV-B as an environmental cue and a potential stressor influencing their growth and development, the specific effects of UV-B-induced oxidative stress on the dynamics of membrane lipids and proteins remain underexplored. Here, we demonstrate that UV-B exposure notably increases the formation of ordered lipid domains on the plasma membrane (PM) and significantly alters the behavior of the Glycine max nodule autoregulation receptor kinase (GmNARK) protein in Arabidopsis leaves. The GmNARK protein was located on the PM and accumulated as small particles in the cytoplasm. We found that UV-B irradiation interrupted the lateral diffusion of GmNARK proteins on the PM. Furthermore, UV-B light decreases the efficiency of surface molecule internalization by clathrin-mediated endocytosis (CME). In brief, UV-B irradiation increased the proportion of the ordered lipid phase and disrupted clathrin-dependent endocytosis; thus, the endocytic trafficking and lateral mobility of GmNARK protein on the plasma membrane are crucial for nodule formation tuning. Our results revealed a novel role of low-intensity UV-B stress in altering the organization of the plasma membrane and the dynamics of membrane-associated proteins.
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页数:13
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