Novel Application of Cultured Epithelial Autografts (CEA) with Expanded Mesh Skin Grafting Over an Artificial Dermis or Dermal Wound Bed Preparation

被引：22

作者：

Akita, Sadanori ^{[1
,2
]}

Hayashida, Kenji ^{[2
,3
]}

Yoshimoto, Hiroshi ^{[2
]}

Fujioka, Masaki ^{[4
]}

Senju, Chikako ^{[2
,4
]}

Morooka, Shin ^{[4
]}

Nishimura, Gozo ^{[5
]}

Mukae, Nobuhiko ^{[6
]}

Kobayashi, Kazuo ^{[7
]}

Anraku, Kuniaki ^{[8
]}

Murakami, Ryuichi ^{[9
]}

Hirano, Akiyoshi ^{[2
]}

Oishi, Masao ^{[2
]}

Ikenoya, Shintaro ^{[10
]}

Amano, Nobuyuki ^{[9
]}

Nakagawa, Hiroshi ^{[7
]}

机构：

[1] Fukuoka Univ, Sch Med, Dept Plast Surg Wound Repair & Regenerat, Fukuoka 8140180, Japan

[2] Nagasaki Univ, Dept Plast & Reconstruct Surg, Grad Sch Biomed Sci, Nagasaki 8528523, Japan

[3] Shimane Univ Hosp, Div Plast & Reconstruct Surg, Izumo, Shimane 6930021, Japan

[4] Natl Hosp Org Nagasaki Med Ctr, Dept Plast & Reconstruct Surg, Nagasaki 8568562, Japan

[5] Fukuoka Tokushukai Hosp, Dept Plast & Reconstruct Surg, Fukuoka 8160864, Japan

[6] Kitakyushu Gen Hosp, Dept Plast & Reconstruct Surg, Kitakyushu, Fukuoka 8028517, Japan

[7] Ehime Prefectural Cent Hosp, Dept Plast & Reconstruct Surg, Matsuyama, Ehime 7900024, Japan

[8] Sasebo City Gen Hosp, Dept Plast & Reconstruct Surg, Sasebo 8570056, Japan

[9] Yamaguchi Prefectural Grand Med Ctr, Dept Plast & Reconstruct Surg, Osaki 7478511, Japan

[10] Matsue Red Cross Hosp, Dept Plast & Reconstruct Surg, Matsue, Shimane 6908506, Japan

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2018年 / 19卷 / 01期

关键词：

split-thickness skin grafting; cultured epithelial autografts (CEA); assessment of scar quality; generalized estimating equation (GEE); generalized linear mixed model (GLMM); EPIDERMAL-CELLS; BURNS; SCAR; RELIABILITY; INJURY; TRIAL;

D O I：

10.3390/ijms19010057

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cultured epithelial autografts (CEA) with highly expanded mesh skin grafts were used for extensive adult burns covering more than 30% of the total body surface area. A prospective study on eight patients assessed subjective and objective findings up to a 12-month follow-up. The results of wound healing for over 1:6 mesh plus CEA, gap 1:6 mesh plus CEA, and 1:3 mesh were compared at 3, 6, and 12 months using extensibility, viscoelasticity, color, and transepidermal water loss by a generalized estimating equation (GEE) or generalized linear mixed model (GLMM). No significant differences were observed among the paired treatments at any time point. At 6 and 12 months, over 1:6 mesh plus CEA achieved significantly better expert evaluation scores by the Vancouver and Manchester Scar Scales (p < 0.01). Extended skin grafting plus CEA minimizes donor resources and the quality of scars is equal or similar to that with conventional low extended mesh slit-thickness skin grafting such as 1:3 mesh. A longitudinal analysis of scars may further clarify the molecular changes of scar formation and pathogenesis.

引用

页数：12

共 23 条

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