Walnut protein isolate-epigallocatechin gallate nanoparticles: A functional carrier enhanced stability and antioxidant activity of lycopene

被引:1
作者
Wang, Yuzhen [1 ]
Lv, Jiao [1 ]
Li, Chang [1 ]
Xu, Yizhuo [1 ]
Jin, Feng [1 ,2 ]
Wang, Fengjun [1 ,2 ]
机构
[1] Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing Key Lab Forest Food Proc & Safety, Natl Key Lab Efficient Prod Forest Resources, Beijing 100083, Peoples R China
[2] 35 Tsinghua East Rd, Beijing 100083, Peoples R China
关键词
Walnut protein isolate; Epigallocatechin gallate; Lycopene; Nanoparticles; Intracellular antioxidant enzyme; Transcriptome; ACTIVITY CAA ASSAY; OXIDATIVE STRESS; BETA-CAROTENE; DELIVERY-SYSTEMS; ANTHERAEA-PERNYI; IDENTIFICATION; ENCAPSULATION; CURCUMIN;
D O I
10.1016/j.foodres.2024.114536
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Walnut isolate protein (WPI)-epigallocatechin gallate (EGCG) conjugates can be employed to creat food-grade delivery systems for preserving bioactive compounds. In this study, WPI-EGCG nanoparticles (WENPs) were developed for encapsulating lycopene (LYC) using the ultrasound-assisted method. The results indicated successful loading of LYC into these WENPs, forming the WENPs/LYC (cylinder with 200-300 nm in length and 14.81-30.05 nm in diameter). Encapsulating LYC in WENPs led to a notable decrease in release rate and improved stability in terms of thermal, ultraviolet (UV), and storage conditions compared to free LYC. Simultaneously, WENPs/LYC exhibited a synergistic and significantly higher antioxidant activity with an EC50 value of 23.98 mu g/mL in HepG2 cells compared to free LYC's 31.54 mu g/mL. Treatment with WENPs/LYC led to a dosedependent restoration of intracellular antioxidant enzyme activities (SOD, CAT, and GSH-Px) and inhibition of intracellular malondialdehyde (MDA) formation. Furthermore, transcriptome analysis indicated that enrichment in glutathione metabolism and peroxisome processes following WENPs/LYC addition. Quantitative real-time reverse transcription PCR (qRT-PCR) verified the expression levels of related genes involved in the antioxidant resistance pathway of WENPs/LYC on AAPH-induced oxidative stress. This study offers novel perspectives into the antioxidant resistance pathway of WENPs/LYC, holding significant potential in food industry.
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页数:14
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