共 31 条
[31]
Zeng Z. G., 2007, CHINESE J SPORTS MED, V26, P120
Determination of β-Hydroxy-β-Methylbutyrate Content in Food Raw Materials by High-Performance Liquid Chromatography
被引：0
作者：
Zhang, Wen-hua
[1
]
Xi, Cunxian
[2
]
Yi, Xionghai
[3
]
Zhang, Yaqin
[1
]
Bao, Weiqi
[1
]
Shao, Li-juan
[4
]
Xu, Dun-ming
[5
]
机构：
[1] Tech Ctr Hangzhou Customs, Hangzhou, Peoples R China
[2] Tech Ctr Chongqing Customs, Chongqing, Peoples R China
[3] Shanghai Customs, Tech Ctr Anim Plant & Food Inspect & Quarantine, Shanghai, Peoples R China
[4] Zhejiang Coll Construct, Hangzhou, Peoples R China
[5] Tech Ctr Xiamen Customs, Xiamen, Peoples R China
基金：
国家重点研发计划;
关键词：
beta-hydroxy-beta-methylbutyrate;
food raw materials;
high-performance liquid chromatography;
METHYL-BUTYRATE;
SUPPLEMENTATION;
COMBINATION;
URINE;
ACIDS;
D O I：
10.1002/sscp.202400144
中图分类号：
O65 [分析化学];
学科分类号：
070302 ;
081704 ;
摘要：
An analytical method based on high-performance liquid chromatography (HPLC) method was developed for the determination of beta-hydroxy-beta-methylbutyrate (HMB) content in food raw materials. The quantitative detection was performed by HPLC on diode array detector mode under external standard method. The limits of quantification, calculated as 10 times the standard deviation, were 2.0 g/100 g. The average recoveries were in the range of 92.3%-101%, with relative standard deviations below 3.87%, measured at three concentration levels. This method was used to determine the purity of HMB in 10 commercially available food raw materials. The real sample analysis results showed that the content and measurement uncertainties of HMB were 77.1-81.8 g/100 g and 4.51-4.68 g/100 g respectively, which met the requirement of HMB content in the range of 77-82 g/100 g in the HMB announcement. There are no studies on the detection of HMB in food raw materials in the previous reports. The established method provided reference for the rapid quantification and purity analysis of beta-hydroxy-beta-methylbutyrate in food raw materials.
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