Delineation of chicken immune markers in the era of omics and multicolor flow cytometry

被引:4
|
作者
Haertle, Sonja [1 ]
Sutton, Kate [2 ]
Vervelde, Lonneke [2 ]
Dalgaard, Tina S. [3 ]
机构
[1] Ludwig Maximilians Univ Munchen, Dept Vet Sci, Munich, Germany
[2] Univ Edinburgh, Roslin Inst, Royal Dick Sch Vet Studies, Div Immunol, Edinburgh, Scotland
[3] Aarhus Univ, Dept Anim & Vet Sci, Tjele, Denmark
基金
欧盟地平线“2020”;
关键词
chicken; leukocytes; flow cytometry; lineages; phenotyping; single-cell biology; DELTA-T-CELLS; NATURAL-KILLER-CELLS; B-CELLS; MONOCLONAL-ANTIBODIES; GENE-EXPRESSION; CELLULAR COMPOSITION; CROSS-REACTIVITY; RECEPTOR COMPLEX; AVIAN INFLUENZA; DENDRITIC CELLS;
D O I
10.3389/fvets.2024.1385400
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Multiparameter flow cytometry is a routine method in immunological studies incorporated in biomedical, veterinary, agricultural, and wildlife research and routinely used in veterinary clinical laboratories. Its use in the diagnostics of poultry diseases is still limited, but due to the continuous expansion of reagents and cost reductions, this may change in the near future. Although the structure and function of the avian immune system show commonalities with mammals, at the molecular level, there is often low homology across species. The cross-reactivity of mammalian immunological reagents is therefore low, but nevertheless, the list of reagents to study chicken immune cells is increasing. Recent improvement in multicolor antibody panels for chicken cells has resulted in more detailed analysis by flow cytometry and has allowed the discovery of novel leukocyte cell subpopulations. In this article, we present an overview of the reagents and guidance needed to perform multicolor flow cytometry using chicken samples and common pitfalls to avoid.
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页数:19
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