Kinetics of plasma apolipoprotein E isoforms by LC-MS/MS: a pilot study

被引:27
作者
Blanchard, Valentin [1 ]
Ramin-Mangata, Stephane [2 ]
Billon-Crossouard, Stephanie [1 ,3 ]
Aguesse, Audrey [1 ,3 ]
Durand, Manon [1 ,4 ]
Chemello, Kevin [2 ]
Nativel, Brice [2 ]
Flet, Laurent [5 ]
Chetiveaux, Maud [1 ]
Jacobi, David [4 ,6 ]
Bard, Jean-Marie [7 ,8 ,9 ]
Ouguerram, Khadija [1 ,3 ]
Lambert, Gilles [2 ]
Krempf, Michel [1 ,3 ,6 ]
Croyal, Mikael [1 ,3 ]
机构
[1] West Human Nutr Res Ctr, CRNHO, F-44000 Nantes, France
[2] Univ Reunion, INSERM, DeTROI, UMR 1188, F-97490 St Clothilde, Reunion, France
[3] INRA, PhAN, UMR 1280, F-44000 Nantes, France
[4] Univ Nantes, Inst Thorax, INSERM, CNRS, F-44000 Nantes, France
[5] Nantes Univ Hosp, Pharm Dept, F-44093 Nantes, France
[6] CHU Nantes, Inst Thorax, F-44093 Nantes, France
[7] Univ Nantes, MMS, EA 2160, F-44000 Nantes, France
[8] IUML, CNRS, FR3473, F-44000 Nantes, France
[9] Inst Canc & Oncol, Dept Biopathol, F-44800 St Herblain, France
关键词
peptide; liquid chromatography; tandem mass spectrometry; stable isotope tracers; lipoprotein/kinetics; lipoprotein/metabolism; TANDEM MASS-SPECTROMETRY; LIPOPROTEIN METABOLISM; APOE GENOTYPE; CHOLESTEROL; DISEASE; MACROPHAGES; LC/MS/MS; RECEPTOR; COMPLEX; PROTEIN;
D O I
10.1194/jlr.P083576
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Human apoE exhibits three major isoforms (apoE2, apoE3, and apoE4) corresponding to polymorphism in the APOE gene. Total plasma apoE concentrations are closely related to these isoforms, but the underlying mechanisms are unknown. We aimed to describe the kinetics of apoE individual isoforms to explore the mechanisms for variable total apoE plasma concentrations. We used LC-MS/MS to discriminate between isoforms by identifying specific peptide sequences in subjects (three E2/E3, three E3/E3, and three E3/E4 phenotypes) who received a primed constant infusion of H-2(3)-leucine for 14 h. apoE concentrations and leucine enrichments were measured hourly in plasma. Concentrations of apoE2 were higher than apoE3, and concentrations of apoE4 were lower than apoE3. There was no difference between apoE3 and apoE4 catabolic rates and between apoE2 and apoE3 production rates (PRs), but apoE2 catabolic rates and apoE4 PRs were lower. The mechanisms leading to the difference in total plasma apoE concentrations are therefore related to contrasted kinetics of the isoforms. Production or catabolic rates are differently affected according to the specific isoforms. On these grounds, studies on the regulation of the involved biochemical pathways and the impact of pathological environments are now warranted.
引用
收藏
页码:892 / 900
页数:9
相关论文
共 38 条
[1]   INTERACTION OF LPB, LPB-E, LPB-C-III, AND LPB-C-III-E LIPOPROTEINS WITH THE LOW-DENSITY-LIPOPROTEIN RECEPTOR OF HELA-CELLS [J].
AGNANI, G ;
BARD, JM ;
CANDELIER, L ;
DELATTRE, S ;
FRUCHART, JC ;
CLAVEY, V .
ARTERIOSCLEROSIS AND THROMBOSIS, 1991, 11 (04) :1021-1029
[2]   Apolipoprotein E kinetics:: influence of insulin resistance and type 2 diabetes [J].
Bach-Ngohou, K ;
Ouguerram, K ;
Nazih, H ;
Maugère, P ;
Ripolles-Piquer, B ;
Zaïr, Y ;
Frénais, R ;
Krempf, M ;
Bard, JM .
INTERNATIONAL JOURNAL OF OBESITY, 2002, 26 (11) :1451-1458
[3]  
Batal R, 2000, J LIPID RES, V41, P706
[4]   REGULATION OF PLASMA-CHOLESTEROL BY LIPOPROTEIN RECEPTORS [J].
BROWN, MS ;
KOVANEN, PT ;
GOLDSTEIN, JL .
SCIENCE, 1981, 212 (4495) :628-635
[5]   Quantification of seven apolipoproteins in human plasma by proteotypic peptides using fast LC-MS/MS [J].
Ceglarek, Uta ;
Dittrich, Julia ;
Becker, Susen ;
Baumann, Frank ;
Kortz, Linda ;
Thiery, Joachim .
PROTEOMICS CLINICAL APPLICATIONS, 2013, 7 (11-12) :794-801
[6]   The differential apoA-I enrichment of preβ1 and αHDL is detectable by gel filtration separation [J].
Chétiveaux, M ;
Nazih, H ;
Ferchaud-Roucher, V ;
Lambert, G ;
Zaïr, Y ;
Masson, M ;
Ouguerram, K ;
Bouhours, D ;
Krempf, M .
JOURNAL OF LIPID RESEARCH, 2002, 43 (11) :1986-1993
[7]   Multiplexed peptide analysis for kinetic measurements of major human apolipoproteins by LC/MS/MS [J].
Croyal, Mikael ;
Fall, Fanta ;
Ferchaud-Roucher, Veronique ;
Chetiveaux, Maud ;
Zair, Yassine ;
Ouguerram, Khadija ;
Krempf, Michel ;
Nobecourt, Estelle .
JOURNAL OF LIPID RESEARCH, 2016, 57 (03) :509-515
[8]   Effects of Extended-Release Nicotinic Acid on Apolipoprotein ( a) Kinetics in Hypertriglyceridemic Patients [J].
Croyal, Mikael ;
Ouguerram, Khadija ;
Passard, Maxime ;
Ferchaud-Roucher, Veronique ;
Chetiveaux, Maud ;
Billon-Crossouard, Stephanie ;
de Gouville, Anne-Charlotte ;
Lambert, Gilles ;
Krempf, Michel ;
Nobecourt, Estelle .
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2015, 35 (09) :2042-2047
[9]   Phenotype-dependent differences in apolipoprotein E metabolism and in cholesterol homeostasis in human monocyte-derived macrophages [J].
Cullen, P ;
Cignarella, A ;
Brennhausen, B ;
Mohr, S ;
Assmann, G ;
von Eckardstein, A .
JOURNAL OF CLINICAL INVESTIGATION, 1998, 101 (08) :1670-1677
[10]   Apolipoproteins: metabolic role and clinical biochemistry applications [J].
Dominiczak, Marek H. ;
Caslake, Muriel J. .
ANNALS OF CLINICAL BIOCHEMISTRY, 2011, 48 :498-515