A Fluorescent Lateral Flow Immunoassay for the Detection of Skeletal Muscle Troponin I in Serum for Muscle Injury Monitoring at the Point of Care

被引：0
|
作者：
Tang, Deding [1 ,2 ,3 ]
Wu, Shuang [1 ,2 ]
Kong, Mengqi [1 ,2 ,4 ]
Liu, Zhaonan [1 ,2 ,4 ]
Li, Zonghao [1 ,2 ,4 ]
Han, Ying [5 ]
Gong, Yan [5 ]
Hu, Jie [4 ]
机构：
[1] Xi An Jiao Tong Univ, Sch Life Sci & Technol, Key Lab Biomed Informat Engn, Minist Educ, Xian 710049, Peoples R China
[2] Xi An Jiao Tong Univ, Bioinspired Engn & Biomech Ctr BEBC, Xian 710049, Peoples R China
[3] Maanshan Teachers Coll, Publ Teaching Dept, Maanshan 243041, Peoples R China
[4] Suzhou Diyinan Biotech Co, Suzhou 215129, Peoples R China
[5] Suzhou Univ Sci & Technol, Sch Chem & Life Sci, Suzhou 215009, Peoples R China
来源：
BIOSENSORS-BASEL | 2024年 / 14卷 / 08期
关键词：
lateral flow immunoassay; sports medicine; exercise-induced muscle injury; point-of-care testing; ASSAY;
D O I：
10.3390/bios14080381
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Exercise-induced muscle injury is one of the most common types of sports injuries. Skeletal muscle troponin I (skTnI) serves as an ideal biomarker in assessing such injuries, facilitating timely detection and evaluation. In this study, we develop a fluorescent sandwich lateral flow immunoassay (LFIA) combined with a desktop analyzer for rapid detection of skTnI. Through optimizing the reaction system, the assay achieves a satisfying detection performance, reaching a limit of detection (LOD) of 0.5 ng/mL with a turnaround time of 15 min. The proposed detection platform offers portability, ease of use, and high sensitivity, which facilitates the monitoring of exercise-induced muscle injuries at the point of care. This feature is particularly advantageous for end users, enabling timely detection of sports-related injuries and ultimately enhancing prognosis and sports life.
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页数：10
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