Exploiting the full potential of cryo-EM maps

被引：3

作者：

Bick, Thomas ^{[1
]}

Dominiak, Paulina M. ^{[2
]}

Wendler, Petra ^{[1
]}

机构：

[1] Univ Potsdam, Inst Biochem & Biol, Dept Biochem, Karl Liebknecht Str 24-25, D-14476 Potsdam Golm, Germany

[2] Univ Warsaw, Fac Chem, Biol & Chem Res Ctr, Ul Zwirki i Wigury 101, PL-02089 Warsaw, Poland

来源：

BBA ADVANCES | 2024年 / 5卷

关键词：

Coulomb potential; Cryo-EM; Charge; Redox proteins; Radiation damage; Fitting algorithms; SCATTERING FACTORS; ELECTRON CRYSTALLOGRAPHY; RADIATION-DAMAGE; IONS; IDENTIFICATION; BACTERIORHODOPSIN; REFINEMENT; DENSITY; COMPLEX; MODEL;

D O I：

10.1016/j.bbadva.2024.100113

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Coulomb potential maps generated by electron microscopy (EM) experiments contain not only information about the position but also about the charge state of the atom. This feature of EM maps allows the identification of specific ions and the protonation state of amino acid side chains in the sample. Here, we summarize qualitative observations of charges in EM maps, discuss the difficulties in interpreting the charge in Coulomb potential maps with respect to distinguishing it from radiation damage, and outline considerations to implement the correct charge in fitting algorithms.

引用

页数：7

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