Blood methylation pattern re fl ects epigenetic remodelling in adipose tissue after bariatric surgery

被引：4

作者：

Mueller, Luise ^{[1
]}

Hoffmann, Anne ^{[2
,3
,7
]}

Bernhart, Stephan H. ^{[4
,5
,6
]}

Ghosh, Adhideb ^{[7
]}

Zhong, Jiawei ^{[8
]}

Hagemann, Tobias ^{[2
,3
]}

Sun, Wenfei ^{[7
]}

Dong, Hua ^{[7
]}

Noe, Falko ^{[7
]}

Wolfrum, Christian ^{[7
]}

Dietrich, Arne ^{[9
]}

Stumvoll, Michael ^{[1
,2
,3
,10
]}

Massier, Lucas ^{[8
]}

Blueher, Matthias ^{[1
,2
,3
,10
]}

Kovacs, Peter ^{[1
,11
]}

Chakaroun, Rima ^{[1
,11
]}

Keller, Maria ^{[1
,2
,3
]}

机构：

[1] Univ Leipzig, Med Dept Endocrinol Nephrol Rheumatol 3, Med Ctr, D-04103 Leipzig, Germany

[2] Univ Leipzig, Univ Hosp Leipzig, Helmholtz Inst Metab Obes & Vasc Res HI MAG, Helmholtz Ctr Munich, Philipp Rosenthal Str 27, D-04103 Leipzig, Germany

[3] Univ Hosp Leipzig, Philipp Rosenthal Str 27, D-04103 Leipzig, Germany

[4] Univ Leipzig, Interdisciplinary Ctr Bioinformat, D-04107 Leipzig, Germany

[5] Univ Leipzig, Dept Comp Sci, Bioinformat Grp, D-04107 Leipzig, Germany

[6] Univ Leipzig, LIFE Res Ctr Civilizat Dis, Transcriptome Bioinformat, D-04107 Leipzig, Germany

[7] Swiss Fed Inst Technol, Inst Food Nutr & Hlth, CH-8092 Schwerzenbach, Switzerland

[8] Karolinska Univ Hosp, Karolinska Inst, Dept Med Huddinge H7, S-14186 Huddinge, Sweden

[9] Leipzig Univ Hosp, Dept Visceral Transplantat Thorac & Vasc Surg, Sect Bariatr Surg, D-04103 Leipzig, Germany

[10] Deutsch Zentrum Diabet Forsch eV, D-85764 Neuherberg, Germany

[11] Univ Gothenburg, Inst Med, Sahlgrenska Acad, Dept Mol & Clin Med,Wallenberg Lab, S-41345 Gothenburg, Sweden

来源：

EBIOMEDICINE | 2024年 / 106卷

基金：

瑞典研究理事会;

关键词：

Bariatric surgery; DNA methylation; Adipose tissue; Blood; In fl ammation; DNA methylation age; BODY-MASS INDEX; DNA METHYLATION; PROMOTER METHYLATION; WEIGHT-LOSS; OBESITY; BCAP; ASSOCIATION; OVERWEIGHT; RESISTANCE; RECEPTOR;

D O I：

10.1016/j.ebiom.2024.105242

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background Studies on DNA methylation following bariatric surgery have primarily focused on blood cells, while it is unclear to which extend it may re fl ect DNA methylation pro fi les in speci fi c metabolically relevant organs such as adipose tissue. Here, we investigated whether adipose tissue depots speci fi c methylation changes after bariatric surgery are mirrored in blood. Methods Using Illumina 850K EPIC technology, we analysed genome-wide DNA methylation in paired blood, subcutaneous and omental visceral AT (SAT/OVAT) samples from nine individuals (N = 6 female) with severe obesity pre- and post-surgery. Findings The numbers and effect sizes of differentially methylated regions (DMRs) post-bariatric surgery were more pronounced in AT (SAT: 12,865 DMRs from - 11.5 to 10.8%; OVAT: 14,632 DMRs from - 13.7 to 12.8%) than in blood (9267 DMRs from - 8.8 to 7.7%). Cross-tissue DMRs implicated immune-related genes. Among them, 49 regions could be validated with similar methylation changes in blood from independent individuals. Fourteen DMRs correlated with differentially expressed genes in AT post bariatric surgery, including downregulation of PIK3AP1 in both SAT and OVAT. DNA methylation age acceleration was signi fi cantly higher in AT compared to blood, but remained unaffected after surgery.

引用

页数：16

共 82 条

[81] More than an Anti-diabetic Bariatric Surgery, Metabolic Surgery Alleviates Systemic and Local Inflammation in Obesity
Zhang, Chunlan
Zhang, Jingjing
Liu, Zhenqi
Zhou, Zhiguang
[J]. OBESITY SURGERY, 2018, 28 (11) : 3658 - 3668
[82] Heavy-tailed prior distributions for sequence count data: removing the noise and preserving large differences
Zhu, Anqi
Ibrahim, Joseph G.
Love, Michael I.
[J]. BIOINFORMATICS, 2019, 35 (12) : 2084 - 2092

← 1 2 3 4 5 6 7 8 9 →