Blood methylation pattern re fl ects epigenetic remodelling in adipose tissue after bariatric surgery

Background Studies on DNA methylation following bariatric surgery have primarily focused on blood cells, while it is unclear to which extend it may re fl ect DNA methylation pro fi les in speci fi c metabolically relevant organs such as adipose tissue. Here, we investigated whether adipose tissue depots speci fi c methylation changes after bariatric surgery are mirrored in blood. Methods Using Illumina 850K EPIC technology, we analysed genome-wide DNA methylation in paired blood, subcutaneous and omental visceral AT (SAT/OVAT) samples from nine individuals (N = 6 female) with severe obesity pre- and post-surgery. Findings The numbers and effect sizes of differentially methylated regions (DMRs) post-bariatric surgery were more pronounced in AT (SAT: 12,865 DMRs from - 11.5 to 10.8%; OVAT: 14,632 DMRs from - 13.7 to 12.8%) than in blood (9267 DMRs from - 8.8 to 7.7%). Cross-tissue DMRs implicated immune-related genes. Among them, 49 regions could be validated with similar methylation changes in blood from independent individuals. Fourteen DMRs correlated with differentially expressed genes in AT post bariatric surgery, including downregulation of PIK3AP1 in both SAT and OVAT. DNA methylation age acceleration was signi fi cantly higher in AT compared to blood, but remained unaffected after surgery.